Cell Biology Antibodies 8

Anti-GDF9 Antibody (CAB2739)

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SKU:
CAB2739
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GDF9 Antibody
Antibody SKU:CAB2739
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 205-454 of human GDF9 (NP_005251.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, 293T, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 205-454 of human GDF9 (NP_005251.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FGKK HKWI QIDV TSLL QPLV ASNK RSIH MSIN FTCM KDQL EHPS AQNG LFNM TLVS PSLI LYLN DTSA QAYH SWYS LHYK RRPS QGPD QERS LSAY PVGE EAAE DGRS SHHR HRRG QETV SSEL KKPL GPAS FNLS EYFR QFLL PQNE CELH DFRL SFSQ LKWD NWIV APHR YNPR YCKG DCPR AVGH RYGS PVHT MVQN IIYE KLDS SVPR PSCV PAKY SPLS VLTI EPDG SIAY KEYE DMIA TKCT CR
Gene ID:2661
Uniprot:O60383
Cellular Location:Secreted
Calculated MW:51kDa
Observed MW:51kDa
Synonyms:GDF9
Background:This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins.
UniProt Protein Function:GDF9: Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins. Belongs to the TGF-beta family.
UniProt Protein Details:

Protein type:Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cytoplasm; extracellular space

Molecular Function:cytokine activity; growth factor activity; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; cell development; female gamete generation; negative regulation of cell growth; oocyte growth; positive regulation of cell proliferation; regulation of apoptosis; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway

NCBI Summary:This gene encodes a member of the transforming growth factor-beta superfamily. The encoded preproprotein is processed into a secreted factor that is required for ovarian folliculogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:O60383
NCBI GenInfo Identifier:6016124
NCBI Gene ID:2661
NCBI Accession:O60383.1
UniProt Secondary Accession:O60383,Q4VAW5,
UniProt Related Accession:O60383
Molecular Weight:51,444 Da
NCBI Full Name:Growth/differentiation factor 9
NCBI Synonym Full Names:growth differentiation factor 9
NCBI Official Symbol:GDF9  
NCBI Protein Information:growth/differentiation factor 9
UniProt Protein Name:Growth/differentiation factor 9
Protein Family:Growth/differentiation factor
UniProt Gene Name:GDF9  
UniProt Entry Name:GDF9_HUMAN
Anti-GDF9 Antibody (CAB2739)Western blot analysis of extracts of various cell lines, using GDF9 antibody (CAB2739) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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