Cell Biology Antibodies 3

Anti-GDF5 Antibody (CAB13167)

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SKU:
CAB13167
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GDF5 Antibody
Antibody SKU:CAB13167
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human GDF5 (NP_000548.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse heart, Rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human GDF5 (NP_000548.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KRDL FFNE IKAR SGQD DKTV YEYL FSQR RKRR APLA TRQG KRPS KNLK ARCS RKAL HVNF KDMG WDDW IIAP LEYE AFHC EGLC EFPL RSHL EPTN HAVI Q
Gene ID:8200
Uniprot:P43026
Cellular Location:Cell membrane, Secreted
Calculated MW:55kDa
Observed MW:45kDa
Synonyms:GDF5, BDA1C, BMP-14, BMP14, CDMP1, LAP-4, LAP4, OS5, SYM1B, SYNS2
Background:This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis.
UniProt Protein Function:GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function:identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: hindlimb morphogenesis; extracellular matrix organization and biogenesis; regulation of multicellular organism growth; regulation of apoptosis; forelimb morphogenesis; BMP signaling pathway; positive regulation of chondrocyte differentiation; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; chondrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; growth

Disease: Chondrodysplasia, Grebe Type; Acromesomelic Dysplasia, Hunter-thompson Type; Brachydactyly, Type A1, C; Brachydactyly, Type A2; Symphalangism, Proximal, 1b; Fibular Hypoplasia And Complex Brachydactyly; Brachydactyly, Type C; Osteoarthritis Susceptibility 5; Multiple Synostoses Syndrome 2

NCBI Summary:The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008]
UniProt Code:P43026
NCBI GenInfo Identifier:20141384
NCBI Gene ID:8200
NCBI Accession:P43026.3
UniProt Secondary Accession:P43026,Q96SB1, E1P5Q2,
UniProt Related Accession:P43026
Molecular Weight:Observed: 55kDaCalculated: 55kDa
NCBI Full Name:Growth/differentiation factor 5
NCBI Synonym Full Names:growth differentiation factor 5
NCBI Official Symbol:GDF5  
NCBI Official Synonym Symbols:OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14  
NCBI Protein Information:growth/differentiation factor 5; CDMP-1; radotermin; LPS-associated protein 4; bone morphogenetic protein 14; lipopolysaccharide-associated protein 4; cartilage-derived morphogenetic protein-1
UniProt Protein Name:Growth/differentiation factor 5
UniProt Synonym Protein Names:Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin
Protein Family:Growth/differentiation factor
UniProt Gene Name:GDF5  
UniProt Entry Name:GDF5_HUMAN
Anti-GDF5 Antibody (CAB13167)Western blot analysis of extracts of various cell lines, using GDF5 antibody (CAB13167) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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