Metabolism Antibodies 2

Anti-GCLM Antibody (CAB5314)

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SKU:
CAB5314
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-GCLM Antibody
Antibody SKU:CAB5314
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:ES-2, U-937, 22Rv1, U-251MG, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGTD SRAA KALL ARAR TLHL QTGN LLNW GRLR KKCP STHS EELH DCIQ KTLN EWSS QINP DLVR EFPD VLEC TVSH AVEK INPD EREE MKVS AKLF IVES NSSS STRS AVDM ACSV LGVA QLDS VIIA SPPI EDGV NLSL EHLQ PYWE ELEN LVQS KKIV AIGT SDLD KTQL EQLY QWAQ VKPN SNQV NLAS CCVM PPDL TAFA KQFD IQLL THND PKEL LSEA SFQE ALQE SIPD IQAH EWVP LWLL RYSV IVKS RGII KSKG YILQ AKRR GS
Gene ID:2730
Uniprot:P48507
Cellular Location:
Calculated MW:28kDa/30kDa
Observed MW:31kDa
Synonyms:GCLM, GLCLR
Background:Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: cytosol; glutamate-cysteine ligase complex

Molecular Function:enzyme regulator activity; glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding

Biological Process: glutamate metabolic process; glutathione biosynthetic process; positive regulation of glutamate-cysteine ligase activity; regulation of blood vessel size; response to drug; response to oxidative stress; sulfur amino acid metabolic process

Disease: Myocardial Infarction, Susceptibility To

NCBI Summary:Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
UniProt Code:P48507
NCBI GenInfo Identifier:1346188
NCBI Gene ID:2730
NCBI Accession:P48507.1
UniProt Secondary Accession:P48507,Q6FHC1, Q9NPX9, Q9NU74, A8K334, D3DT45, M5A959
UniProt Related Accession:P48507
Molecular Weight:28,135 Da
NCBI Full Name:Glutamate--cysteine ligase regulatory subunit
NCBI Synonym Full Names:glutamate-cysteine ligase modifier subunit
NCBI Official Symbol:GCLM  
NCBI Official Synonym Symbols:GLCLR  
NCBI Protein Information:glutamate--cysteine ligase regulatory subunit
UniProt Protein Name:Glutamate--cysteine ligase regulatory subunit
UniProt Synonym Protein Names:GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit
Protein Family:Glutamate--cysteine ligase regulatory
UniProt Gene Name:GCLM  
UniProt Entry Name:GSH0_HUMAN
Anti-GCLM Antibody (CAB5314)Western blot analysis of extracts of various cell lines, using GCLM antibody (CAB5314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-GCLM Antibody (CAB5314)Immunofluorescence analysis of U2OS cells using GCLM antibody (CAB5314). Blue: DAPI for nuclear staining.
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