Metabolism Antibodies 1
Anti-GCLC Antibody (CAB1038)
- SKU:
- CAB1038
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-GCLC Antibody |
| Antibody SKU: | CAB1038 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:3000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Mouse kidney, Mouse liver, Mouse lung, Rat kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGLL SQGS PLSW EETK RHAD HVRR HGIL QFLH IYHA VKDR HKDV LKWG DEVE YMLV SFDH ENKK VRLV LSGE KVLE TLQE KGER TNPN HPTL WRPE YGSY MIEG TPGQ PYGG TMSE FNTV EANM RKRR KEAT SILE ENQA LCTI TSFP RLGC PGFT LPEV KPNP VEGG ASKS LFFP DEAI NKHP RFST LTRN IRHR RGEK VVIN VPIF KDKN TPSP FIET FTED DEAS RASK PDHI YMDA MGFG MGNC CLQV |
| Gene ID: | 2729 |
| Uniprot: | P48506 |
| Cellular Location: | |
| Calculated MW: | 72kDa |
| Observed MW: | 72kDa |
| Synonyms: | GCLC, GCL, GCS, GLCL, GLCLC |
| Background: | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. |
| UniProt Protein Function: | GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family. |
| UniProt Protein Details: | Protein type:Ligase; Other Amino Acids Metabolism - glutathione; EC 6.3.2.2 Chromosomal Location of Human Ortholog: 6p12 Cellular Component: glutamate-cysteine ligase complex; cytosol Molecular Function:glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; coenzyme binding; ADP binding; ATP binding Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; sulfur amino acid metabolic process; response to heat; cell redox homeostasis; xenobiotic metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of transcription, DNA-dependent; regulation of blood vessel size; regulation of mitochondrial depolarization; negative regulation of apoptosis Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To |
| NCBI Summary: | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010] |
| UniProt Code: | P48506 |
| NCBI GenInfo Identifier: | 1346190 |
| NCBI Gene ID: | 2729 |
| NCBI Accession: | P48506.2 |
| UniProt Secondary Accession: | P48506,Q14399, |
| UniProt Related Accession: | P48506 |
| Molecular Weight: | 72,766 Da |
| NCBI Full Name: | Glutamate--cysteine ligase catalytic subunit |
| NCBI Synonym Full Names: | glutamate-cysteine ligase, catalytic subunit |
| NCBI Official Symbol: | GCLC |
| NCBI Official Synonym Symbols: | GCL; GCS; GLCL; GLCLC |
| NCBI Protein Information: | glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase |
| UniProt Protein Name: | Glutamate--cysteine ligase catalytic subunit |
| UniProt Synonym Protein Names: | GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase |
| Protein Family: | Glutamate--cysteine ligase |
| UniProt Gene Name: | GCLC |
| UniProt Entry Name: | GSH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GCLC antibody (CAB1038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of L929 cells using GCLC Rabbit pAb (CAB1038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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