Metabolism Antibodies 2

Anti-GCK Antibody (CAB6293)

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SKU:
CAB6293
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-GCK Antibody
Antibody SKU:CAB6293
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCK (NP_000153.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCK (NP_000153.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLDD RARM EAAK KEKV EQIL AEFQ LQEE DLKK VMRR MQKE MDRG LRLE THEE ASVK MLPT YVRS TPEG SEVG DFLS LDLG GTNF RVML VKVG EGEE GQWS VKTK HQMY SIPE DAMT GTAE MLFD YISE CISD FLDK HQMK HKKL PLGF TFSF PVRH EDID KGIL LNWT KGFK ASGA EGNN VVGL LRDA IKRR GDFE MDVV AMVN DTVA TMIS CYYE DHQC EVGM IVGT GCNA CYME EMQN VELV EGDE GRMC VNTE WGAF GDSG ELDE FLLE YDRL VDES SANP GQQL YEKL IGGK YMGE
Gene ID:2645
Uniprot:P35557
Cellular Location:Cytoplasm, Nucleus
Calculated MW:52kDa
Observed MW:52kDa
Synonyms:GCK, FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2
Background:Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
UniProt Code:P35557
NCBI GenInfo Identifier:547696
NCBI Gene ID:2645
NCBI Accession:P35557.1
UniProt Secondary Accession:P35557,Q05810, A4D2J2, A4D2J3,
UniProt Related Accession:P35557
Molecular Weight:465
NCBI Full Name:Glucokinase
NCBI Synonym Full Names:glucokinase (hexokinase 4)
NCBI Official Symbol:GCK  
NCBI Official Synonym Symbols:GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3  
NCBI Protein Information:glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase
UniProt Protein Name:Glucokinase
UniProt Synonym Protein Names:Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
UniProt Gene Name:GCK  
UniProt Entry Name:HXK4_HUMAN
Anti-GCK Antibody (CAB6293)Western blot analysis of extracts of various cell lines, using GCK antibody (CAB6293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-GCK Antibody (CAB6293)Immunofluorescence analysis of U2OS cells using GCK antibody (CAB6293). Blue: DAPI for nuclear staining.
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