Name: Anti-GCC2 Antibody (CAB13814)
Brand: Cell Biology Antibodies 4
SKU: CAB13814
Availability: OutOfStock

Antibody Name:	Anti-GCC2 Antibody
Antibody SKU:	CAB13814
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, LO2, 22Rv1, Raji, SGC-7901, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEDL VQDG VASP ATPG TGKS KLET LPKE DLIK FAKK QMML IQKA KSRC TELE KEIE ELRS KPVT EGTG DIIK ALTE RLDA LLLE KAET EQQC LSLK KENI KMKQ EVED SVTK MGDA HKEL EQSH INYV KEIE NLKN ELMA VRSK YSED KANL QKQL EEAM NTQL ELSE QLKF QNNS EDNV KKLQ EEIE KIRP GFEE QILY LQKQ LDAT TDEK KETV TQLQ NIIE ANSQ HYQK NINS LQEE LLQL KAIH QEEV KELM CQIE ASAK EHEA EINK LNEL KENL VKQC EASE KNIQ KKYE CELE
Gene ID:	9648
Uniprot:	Q8IWJ2
Cellular Location:	Cytoplasm, Golgi apparatus, Peripheral membrane protein, trans-Golgi network membrane
Calculated MW:	5kDa/195kDa
Observed MW:	196kDa

Synonyms:	GCC2, GCC185, RANBP2L4, REN53
Background:	The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GCC2: Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, peripheral Chromosomal Location of Human Ortholog: 2q12.3 Cellular Component: cytosol; Golgi apparatus; membrane; trans-Golgi network Molecular Function:identical protein binding; protein binding Biological Process: Golgi to plasma membrane protein transport; microtubule organizing center organization and biogenesis; protein localization in Golgi apparatus; protein targeting to Golgi; protein targeting to lysosome; retrograde transport, endosome to Golgi
NCBI Summary:	The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Code:	Q8IWJ2
NCBI GenInfo Identifier:	7662062
NCBI Gene ID:	9648
NCBI Accession:	NP_055450.1
UniProt Secondary Accession:	Q8IWJ2,O15045, Q4ZG46, Q8TDH3, Q9H2G8, A6H8X8,
UniProt Related Accession:	Q8IWJ2
Molecular Weight:	5,043 Da
NCBI Full Name:
NCBI Synonym Full Names:	GRIP and coiled-coil domain containing 2
NCBI Official Symbol:	GCC2
NCBI Official Synonym Symbols:	REN53; GCC185; RANBP2L4
NCBI Protein Information:	GRIP and coiled-coil domain-containing protein 2
UniProt Protein Name:	GRIP and coiled-coil domain-containing protein 2
UniProt Synonym Protein Names:	185 kDa Golgi coiled-coil protein; GCC185; CLL-associated antigen KW-11; CTCL tumor antigen se1-1; Ran-binding protein 2-like 4; RanBP2L4; Renal carcinoma antigen NY-REN-53
UniProt Gene Name:	GCC2
UniProt Entry Name:	GCC2_HUMAN

	Western blot analysis of extracts of various cell lines, using GCC2 antibody (CAB13814) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody (CAB13814) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody (CAB13814) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody (CAB13814) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using GCC2 antibody (CAB13814) at dilution of 1:150 (40x lens).