Antibody Name:	Anti-GCAT Antibody
Antibody SKU:	CAB15802
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-419 of human GCAT (NP_055106.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-419 of human GCAT (NP_055106.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KAHK YRYR HLDM ADLE AKLQ EAQK HRLR LVAT DGAF SMDG DIAP LQEI CCLA SRYG ALVF MDEC HATG FLGP TGRG TDEL LGVM DQVT IINS TLGK ALGG ASGG YTTG PGPL VSLL RQRA RPYL FSNS LPPA VVGC ASKA LDLL MGSN TIVQ SMAA KTQR FRSK MEAA GFTI SGAS HPIC PVML GDAR LASR MADD MLKR GIFV IGFS YPVV PKGK ARIR VQIS AVHS EEDI DRCV EAFV EVGR LHGA LP
Gene ID:	23464
Uniprot:	O75600
Cellular Location:	Mitochondrion, Nucleus
Calculated MW:	45kDa/47kDa
Observed MW:	45kDa

Synonyms:

GCAT, KBL

Background:

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.

UniProt Protein Function:	GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
UniProt Protein Details:	Protein type:Transferase; Amino Acid Metabolism - glycine, serine and threonine; EC 2.3.1.29; Mitochondrial Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: mitochondrial inner membrane; mitochondrion; nucleoplasm Biological Process: threonine catabolic process to pyruvate
NCBI Summary:	The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
UniProt Code:	O75600
NCBI GenInfo Identifier:	6685545
NCBI Gene ID:	23464
NCBI Accession:	O75600.1
UniProt Secondary Accession:	O75600,Q6ZWF1, Q96CA9, E2QC23,
UniProt Related Accession:	O75600
Molecular Weight:	47,974 Da
NCBI Full Name:	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
NCBI Synonym Full Names:	glycine C-acetyltransferase
NCBI Official Symbol:	GCAT
NCBI Official Synonym Symbols:	KBL
NCBI Protein Information:	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Protein Name:	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Synonym Protein Names:	Aminoacetone synthase; Glycine acetyltransferase
UniProt Gene Name:	GCAT
UniProt Entry Name:	KBL_HUMAN

	Western blot analysis of extracts of mouse eye, using GCAT antibody (CAB15802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using GCAT antibody (CAB15802) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using GCAT antibody (CAB15802) at dilution of 1:100. Blue: DAPI for nuclear staining.