Cell Biology Antibodies 10
Anti-GCA Antibody (CAB7086)
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- SKU:
- CAB7086
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GCA Antibody |
| Antibody SKU: | CAB7086 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GCA (NP_036330.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, HT-29, Mouse kidney, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GCA (NP_036330.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAYP GYGG GFGN FSIQ VPGM QMGQ PVPE TGPA ILLD GYSG PAYS DTYS SAGD SVYT YFSA VAGQ DGEV DAEE LQRC LTQS GING TYSP FSLE TCRI MIAM LDRD HTGK MGFN AFKE LWAA LNAW KENF MTVD QDGS GTVE HHEL RQAI GLMG YRLS PQTL TTIV KRYS KNGR IFFD DYVA CCVK LRAL TDFF RKRD HLQQ GSAN FIYD DFLQ GTMA I |
| Gene ID: | 25801 |
| Uniprot: | P28676 |
| Cellular Location: | Cytoplasm, Cytoplasmic granule membrane, Cytoplasmic side, Peripheral membrane protein |
| Calculated MW: | 24kDa |
| Observed MW: | 24kDa |
| Synonyms: | GCA, GCL |
| Background: | This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. |
| UniProt Protein Function: | GCA: Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions. |
| UniProt Protein Details: | Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 2q24.2 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein homodimerization activity; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding Biological Process: proteolysis |
| NCBI Summary: | This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P28676 |
| NCBI GenInfo Identifier: | 1170014 |
| NCBI Gene ID: | 25801 |
| NCBI Accession: | P28676.2 |
| UniProt Secondary Accession: | P28676,Q53TB5, Q59EP3, B2R5X3, |
| UniProt Related Accession: | P28676 |
| Molecular Weight: | 217 |
| NCBI Full Name: | Grancalcin |
| NCBI Synonym Full Names: | grancalcin, EF-hand calcium binding protein |
| NCBI Official Symbol: | GCA |
| NCBI Official Synonym Symbols: | GCL |
| NCBI Protein Information: | grancalcin; grancalcin, penta-EF-hand protein; grancalcin, EF-hand calcium-binding protein |
| UniProt Protein Name: | Grancalcin |
| Protein Family: | Grancalcin |
| UniProt Gene Name: | GCA |
| UniProt Entry Name: | GRAN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GCA antibody (CAB7086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A549 cells using GCA antibody (CAB7086). |
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