Immunology Antibodies 1

Anti-GBF1 Antibody (CAB12539)

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SKU:
CAB12539
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-GBF1 Antibody
Antibody SKU:CAB12539
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVDK NIYI IQGE INIV VGAI KRNA RWST HTPL DEER DPLL HSFG HLKE VLNS ITEL SEIE PNVF LRPF LEVI RSED TTGP ITGL A
Gene ID:8729
Uniprot:Q92538
Cellular Location:Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Lipid droplet, Membrane, Peripheral membrane protein, cis-Golgi network, trans-Golgi network
Calculated MW:206kDa
Observed MW:260kDa
Synonyms:GBF1, ARF1GEF
Background:This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.
UniProt Protein Function:Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmatic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5. Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891).
NCBI Summary:This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]
UniProt Code:Q92538
NCBI GenInfo Identifier:13124260
NCBI Gene ID:8729
NCBI Accession:Q92538.2
UniProt Secondary Accession:Q92538,Q149P0, Q149P1, Q5VXX3, Q96CK6, Q96HZ3, Q9H473
UniProt Related Accession:Q92538
Molecular Weight:206kDa
NCBI Full Name:Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
NCBI Synonym Full Names:golgi brefeldin A resistant guanine nucleotide exchange factor 1
NCBI Official Symbol:GBF1  
NCBI Official Synonym Symbols:ARF1GEF  
NCBI Protein Information:Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
UniProt Protein Name:Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Protein Family:G-box-binding factor
UniProt Gene Name:GBF1  
Anti-GBF1 Antibody (CAB12539)Western blot analysis of extracts of HeLa cells, using GBF1 Antibody (CAB12539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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