Cell Biology Antibodies 11
Anti-GBA3 Antibody (CAB7827)
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- SKU:
- CAB7827
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GBA3 Antibody |
| Antibody SKU: | CAB7827 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human GBA3 (NP_001121904.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, HepG2, SW480, Raji, A-549, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human GBA3 (NP_001121904.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAFP AGFG WAAA TAAY QVEG GWDA DGKG PCVW DTFT HQGG ERVF KNQT GDVA CGSY TLWE EDLK CIKQ LGLT HYRF SLSW SRLL PDGT TGFI NQKA IQLD KVNL QVYC AWSL LDNF EWNQ GYSS RFGL FHVD FEDP ARPR VPYT SAKE YAKI IRNN GLEA HL |
| Gene ID: | 57733 |
| Uniprot: | Q9H227 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 18kDa/53kDa |
| Observed MW: | 54kDa |
| Synonyms: | GBA3, CBG, CBGL1, GLUC, KLRP |
| Background: |
| UniProt Protein Function: | GBA3: Glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta- glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - starch and sucrose; Hydrolase; EC 3.2.1.21; Other Amino Acids Metabolism - cyanoamino acid Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: cytosol Molecular Function:beta-galactosidase activity; beta-glucosidase activity; glucosylceramidase activity; glycosylceramidase activity Biological Process: glycoside catabolic process; glycosphingolipid metabolic process; glycosylceramide catabolic process |
| NCBI Summary: | The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
| UniProt Code: | Q9H227 |
| NCBI GenInfo Identifier: | 77416427 |
| NCBI Gene ID: | 57733 |
| NCBI Accession: | Q9H227.2 |
| UniProt Secondary Accession: | Q9H227,Q32LY7, Q3MIH4, Q53GG8, Q6NSF4, Q8NHT8, Q9H3T4 Q9H4C6, |
| UniProt Related Accession: | Q9H227 |
| Molecular Weight: | 18,265 Da |
| NCBI Full Name: | Cytosolic beta-glucosidase |
| NCBI Synonym Full Names: | glucosylceramidase beta 3 (gene/pseudogene) |
| NCBI Official Symbol: | GBA3 |
| NCBI Official Synonym Symbols: | CBG; GLUC; KLRP; CBGL1 |
| NCBI Protein Information: | cytosolic beta-glucosidase |
| UniProt Protein Name: | Cytosolic beta-glucosidase |
| UniProt Synonym Protein Names: | Cytosolic beta-glucosidase-like protein 1 |
| Protein Family: | Cytosolic beta-glucosidase |
| UniProt Gene Name: | GBA3 |
| UniProt Entry Name: | GBA3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GBA3 antibody (CAB7827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A549 cells using GBA3 antibody (CAB7827). Blue: DAPI for nuclear staining. |
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