Metabolism Antibodies 1

Anti-GBA2 Antibody (CAB12834)

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SKU:
CAB12834
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-GBA2 Antibody
Antibody SKU:CAB12834
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 708-927 of human GBA2 (NP_065995.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 708-927 of human GBA2 (NP_065995.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AQDI QDKF SSIL SRGQ EAYE RLLW NGRY YNYD SSSR PQSR SVMS DQCA GQWF LKAC GLGE GDTE VFPT QHVV RALQ TIFE LNVQ AFAG GAMG AVNG MQPH GVPD KSSV QSDE VWVG VVYG LAAT MIQE GLTW EGFQ TAEG CYRT VWER LGLA FQTP EAYC QQRV FRSL AYMR PLSI WAMQ LALQ QQQH KKAS WPKV KQGT GLRT GPMF GPKE AMAN LSPE
Gene ID:57704
Uniprot:Q9HCG7
Cellular Location:Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:72kDa/98kDa/104kDa
Observed MW:105kDa
Synonyms:GBA2, AD035, NLGase, SPG46
Background:This gene encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides as endogenous compounds. Studies to determine subcellular localization of this protein in the liver indicated that the enzyme was mainly enriched in the microsomal fraction where it appeared to be confined to the endoplasmic reticulum. This putative transmembrane protein is thought to play a role in carbohydrate transport and metabolism.
UniProt Protein Function:GBA2: Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide. Involved in sphingomyelin generation and prevention of glycolipid accumulation. May also catalyze the hydrolysis of bile acid 3-O- glucosides, however, the relevance of such activity is unclear in vivo. Belongs to the non-lysosomal glucosylceramidase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.2.1.45; Endoplasmic reticulum; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: integral to membrane; plasma membrane; smooth endoplasmic reticulum

Molecular Function:beta-glucosidase activity; glucosylceramidase activity

Biological Process: bile acid metabolic process; central nervous system neuron development; glycoside catabolic process; glycosphingolipid metabolic process

Disease: Spastic Paraplegia 46, Autosomal Recessive

NCBI Summary:This gene encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides as endogenous compounds. Studies to determine subcellular localization of this protein in the liver indicated that the enzyme was mainly enriched in the microsomal fraction where it appeared to be confined to the endoplasmic reticulum. This putative transmembrane protein is thought to play a role in carbohydrate transport and metabolism. [provided by RefSeq, Jul 2008]
UniProt Code:Q9HCG7
NCBI GenInfo Identifier:143018392
NCBI Gene ID:57704
NCBI Accession:Q9HCG7.2
UniProt Secondary Accession:Q9HCG7,Q5TCV6, Q96A51, Q96LY1, Q96SJ2, Q9H2L8, D3DRP2
UniProt Related Accession:Q9HCG7
Molecular Weight:105kDa
NCBI Full Name:Non-lysosomal glucosylceramidase
NCBI Synonym Full Names:glucosylceramidase beta 2
NCBI Official Symbol:GBA2  
NCBI Official Synonym Symbols:AD035; SPG46; NLGase  
NCBI Protein Information:non-lysosomal glucosylceramidase
UniProt Protein Name:Non-lysosomal glucosylceramidase
UniProt Synonym Protein Names:Beta-glucocerebrosidase 2; Beta-glucosidase 2; Glucosylceramidase 2
UniProt Gene Name:GBA2  
Anti-GBA2 Antibody (CAB12834)Western blot analysis of extracts of various cell lines, using GBA2 antibody (CAB12834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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