Metabolism Antibodies 1
Anti-GATC Antibody (CAB14165)
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- SKU:
- CAB14165
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-GATC Antibody |
| Antibody SKU: | CAB14165 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human GATC (NP_789788.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human GATC (NP_789788.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MWSR LVWL GLRA PLGG RQGF TSKA DPQG SGRI TAAV IEHL ERLA LVDF GSRE AVAR LEKA IAFA DRLR AVDT DGVE PMES VLED RCLY LRSD NVVE GNCA DELL QNSH RVVE EYFV APPG NISL PKLD EQEP FPHS |
| Gene ID: | 283459 |
| Uniprot: | O43716 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 15kDa |
| Observed MW: | 14kDa |
| Synonyms: | GATC, 15E1.2 |
| Background: |
| UniProt Protein Function: | GATC: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the GatC family. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 6.3.5.- Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: mitochondrion Molecular Function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; protein binding Biological Process: mitochondrial translation |
| UniProt Code: | O43716 |
| NCBI GenInfo Identifier: | 76828060 |
| NCBI Gene ID: | 283459 |
| NCBI Accession: | BC107145 |
| UniProt Secondary Accession: | O43716,Q3B824, Q3KNR8, B3KSU7, |
| UniProt Related Accession: | O43716 |
| Molecular Weight: | 15,086 Da |
| NCBI Full Name: | Homo sapiens glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial), mRNA |
| NCBI Synonym Full Names: | glutamyl-tRNA amidotransferase subunit C |
| NCBI Official Symbol: | GATC |
| NCBI Official Synonym Symbols: | 15E1.2 |
| NCBI Protein Information: | glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial |
| UniProt Protein Name: | Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial |
| UniProt Synonym Protein Names: | Protein 15E1.2 |
| Protein Family: | PTS system galactitol-specific EIIC component |
| UniProt Gene Name: | GATC |
| UniProt Entry Name: | GATC_HUMAN |
 | Western blot analysis of extracts of MCF7 cells, using GATC antibody (CAB14165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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