Immunology Antibodies 2
Anti-GATA3 Antibody (CAB1638)
- SKU:
- CAB1638
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-GATA3 Antibody |
| Antibody SKU: | CAB1638 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GATA3 (NP_001002295.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, mouse kidney, mouse brain, rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GATA3 (NP_001002295.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEVT ADQP RWVS HHHP AVLN GQHP DTHH PGLS HSYM DAAQ YPLP EEVD VLFN IDGQ GNHV PPYY GNSV RATV QRYP PTHH GSQV CRPP LLHG SLPW LDGG KALG SHHT ASPW NLSP FSKT SIHH GSPG PLSV YPPA SSSS LSGG HASP HLFT FPPT PPKD VSPD PSLS TPGS AGSA RQDE KECL KYQV PLPD SMKL ESSH SRGS MTAL GGAS SSTH HPIT TYPP YVPE YSSG LFPP SSLL GGSP TGFG CKSR PKAR SSTE |
| Gene ID: | 2625 |
| Uniprot: | P23771 |
| Cellular Location: | Nucleus |
| Calculated MW: | 47kDa/48kDa |
| Observed MW: | 48kDa |
| Synonyms: | GATA3, HDR, HDRS |
| Background: | This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. |
| UniProt Protein Function: | GATA3: a transcriptional regulator central in Th2 cell differentiation. Inhibits breast cancer growth and pulmonary breast cancer metastasis. Represses INK4C transcription, constrains luminal progenitor cell expansion, and suppresses luminal tumorigenesis in the mammary gland. High GATA3 and low INK4C expression predicts a favorable patient outcome in luminal A type breast tumors. IFN-lambda1 (IL-29) inhibits GATA3 expression and suppresses Th2 responses in human T cells. GATA3 haploinsufficiency leads to HDR (hypoparathyroidism, deafness, and renal dysplasia) syndrome. Two alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 10p15 Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus Molecular Function:protein dimerization activity; protein binding; DNA binding; zinc ion binding; interleukin-2 receptor binding; transcription coactivator activity; transcription factor binding; transcription factor activity Biological Process: developmental growth; positive regulation of transcription, DNA-dependent; cell maturation; ear development; cell fate determination; uterus development; T cell receptor signaling pathway; post-embryonic development; negative regulation of interleukin-2 production; norepinephrine biosynthetic process; regulation of neuron apoptosis; erythrocyte differentiation; anatomical structure formation; mesonephros development; kidney development; regulation of cytokine biosynthetic process; response to drug; thymic T cell selection; anatomical structure morphogenesis; inner ear morphogenesis; positive regulation of signal transduction; embryonic hemopoiesis; response to virus; negative regulation of fat cell differentiation; parathyroid gland development; negative regulation of mammary gland epithelial cell proliferation; response to ethanol; positive regulation of T cell differentiation; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; pro-T cell differentiation; negative regulation of transcription, DNA-dependent; regulation of CD4-positive, alpha beta T cell differentiation; transcription from RNA polymerase II promoter; lens development in camera-type eye; axon guidance; phosphoinositide 3-kinase cascade; TOR signaling pathway; neuron migration; defense response; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of cell cycle; positive regulation of interleukin-4 production; negative regulation of cell proliferation; sympathetic nervous system development; negative regulation of interferon-gamma production; response to gamma radiation; type IV hypersensitivity; pharyngeal system development; thymus development; in utero embryonic development; male gonad development; humoral immune response; positive regulation of protein kinase B signaling cascade; negative regulation of inflammatory response; innate immune response; regulation of histone H3-K4 methylation; T-helper 2 cell differentiation; blood coagulation Disease: Hypoparathyroidism, Sensorineural Deafness, And Renal Disease |
| NCBI Summary: | This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009] |
| UniProt Code: | P23771 |
| NCBI GenInfo Identifier: | 120962 |
| NCBI Gene ID: | 2625 |
| NCBI Accession: | P23771.1 |
| UniProt Secondary Accession: | P23771,Q5VWG7, Q5VWG8, Q96J16, |
| UniProt Related Accession: | P23771 |
| Molecular Weight: | 443 |
| NCBI Full Name: | Trans-acting T-cell-specific transcription factor GATA-3 |
| NCBI Synonym Full Names: | GATA binding protein 3 |
| NCBI Official Symbol: | GATA3 |
| NCBI Official Synonym Symbols: | HDR; HDRS |
| NCBI Protein Information: | trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3 |
| UniProt Protein Name: | Trans-acting T-cell-specific transcription factor GATA-3 |
| UniProt Synonym Protein Names: | GATA-binding factor 3 |
| Protein Family: | GATA-binding factor |
| UniProt Gene Name: | GATA3 |
| UniProt Entry Name: | GATA3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GATA3 antibody (CAB1638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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