Name: Anti-GAR1 Antibody (CAB12748)
Brand: Cell Biology Antibodies 3
SKU: CAB12748
Availability: OutOfStock

Antibody Name:	Anti-GAR1 Antibody
Antibody SKU:	CAB12748
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human GAR1 (NP_061856.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human GAR1 (NP_061856.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KGQD QGPP ERVV LLGE FLHP CEDD IVCK CTTD ENKV PYFN APVY LENK EQIG KVDE IFGQ LRDF YFSV KLSE NMKA SSFK KLQK FYID PYKL LPLQ RFLP RPPG EKGP PRG
Gene ID:	54433
Uniprot:	Q9NY12
Cellular Location:	Cajal body, Nucleus, nucleolus
Calculated MW:	20kDa/22kDa
Observed MW:	28kDa

Synonyms:

GAR1, NOLA1

Background:

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.

UniProt Protein Function:	NOLA1: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Belongs to the GAR1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleolus; RNA processing; RNA-binding Chromosomal Location of Human Ortholog: 4q25 Cellular Component: fibrillar center; nuclear chromosome, telomeric region; nucleoplasm; nucleus; telomerase holoenzyme complex Molecular Function:protein binding; RNA binding Biological Process: rRNA pseudouridine synthesis; snoRNA guided rRNA pseudouridine synthesis; telomere maintenance via telomerase
NCBI Summary:	This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NY12
NCBI GenInfo Identifier:	51828015
NCBI Gene ID:	54433
NCBI Accession:	Q9NY12.1
UniProt Secondary Accession:	Q9NY12,Q5MJQ2,
UniProt Related Accession:	Q9NY12
Molecular Weight:	22kDa
NCBI Full Name:	H/ACA ribonucleoprotein complex subunit 1
NCBI Synonym Full Names:	GAR1 ribonucleoprotein
NCBI Official Symbol:	GAR1
NCBI Official Synonym Symbols:	NOLA1
NCBI Protein Information:	H/ACA ribonucleoprotein complex subunit 1
UniProt Protein Name:	H/ACA ribonucleoprotein complex subunit 1
UniProt Synonym Protein Names:	Nucleolar protein family A member 1; snoRNP protein GAR1
Protein Family:	D-galacturonate reductase
UniProt Gene Name:	GAR1

Western blot analysis of extracts of various cell lines, using GAR1 antibody (CAB12748) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

GAR1 Antibody (PACO09413)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)