Cell Biology Antibodies 10
Anti-Gamma-Tubulin Antibody (CAB6215)
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- SKU:
- CAB6215
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Gamma-Tubulin Antibody |
| Antibody SKU: | CAB6215 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 152-451 of human Gamma-Tubulin (NP_001061.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 152-451 of human Gamma-Tubulin (NP_001061.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YLLE RLND RYPK KLVQ TYSV FPNQ DEMS DVVV QPYN SLLT LKRL TQNA DCVV VLDN TALN RIAT DRLH IQNP SFSQ INQL VSTI MSAS TTTL RYPG YMNN DLIG LIAS LIPT PRLH FLMT GYTP LTTD QSVA SVRK TTVL DVMR RLLQ PKNV MVST GRDR QTNH CYIA ILNI IQGE VDPT QVHK SLQR IRER KLAN FIPW GPAS IQVA LSRK SPYL PSAH RVSG LMMA NHTS ISSL FERT CRQY DKLR KREA FLEQ FRKE DMFK DNFD EMDT SREI VQQL IDEY HAAT RPDY ISWG TQEQ |
| Gene ID: | 7283 |
| Uniprot: | P23258 |
| Cellular Location: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 51kDa |
| Observed MW: | 51kDa |
| Synonyms: | TUBG1, CDCBM4, GCP-1, TUBG, TUBGCP1 |
| Background: | This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. |
| UniProt Protein Function: | TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q21 Cellular Component: centriole; centrosome; pericentriolar material; recycling endosome; condensed nuclear chromosome; apical part of cell; cytoplasmic microtubule; nonmotile primary cilium; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton Biological Process: metabolic process; organelle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule nucleation; meiotic spindle organization and biogenesis; cytoplasmic microtubule organization and biogenesis Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4 |
| NCBI Summary: | This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009] |
| UniProt Code: | P23258 |
| NCBI GenInfo Identifier: | 20455518 |
| NCBI Gene ID: | 7283 |
| NCBI Accession: | P23258.2 |
| UniProt Secondary Accession: | P23258,Q53X79, Q9BW59, |
| UniProt Related Accession: | P23258 |
| Molecular Weight: | 451 |
| NCBI Full Name: | Tubulin gamma-1 chain |
| NCBI Synonym Full Names: | tubulin, gamma 1 |
| NCBI Official Symbol: | TUBG1 |
| NCBI Official Synonym Symbols: | TUBG; GCP-1; CDCBM4; TUBGCP1 |
| NCBI Protein Information: | tubulin gamma-1 chain; tubulin, gamma polypeptide; gamma-tubulin complex component 1 |
| UniProt Protein Name: | Tubulin gamma-1 chain |
| UniProt Synonym Protein Names: | Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1 |
| UniProt Gene Name: | TUBG1 |
| UniProt Entry Name: | TBG1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using gamma-Tubulin antibody (CAB6215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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