Cell Biology Antibodies 5
Anti-Gamma-Tubulin Antibody (CAB14554)
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- SKU:
- CAB14554
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Gamma-Tubulin Antibody |
| Antibody SKU: | CAB14554 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Gamma-Tubulin (NP_001061.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HeLa, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Gamma-Tubulin (NP_001061.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PWGP ASIQ VALS RKSP YLPS AHRV SGLM MANH TSIS SLFE RTCR QYDK LRKR EAFL EQFR KEDM FKDN FDEM DTSR EIVQ QLID EYHA ATRP DYIS WGTQ E |
| Gene ID: | 7283 |
| Uniprot: | P23258 |
| Cellular Location: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 51kDa |
| Observed MW: | 51kDa |
| Synonyms: | TUBG1, CDCBM4, GCP-1, TUBG, TUBGCP1 |
| Background: | This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. |
| UniProt Protein Function: | TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q21 Cellular Component: centriole; centrosome; pericentriolar material; recycling endosome; condensed nuclear chromosome; apical part of cell; cytoplasmic microtubule; nonmotile primary cilium; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton Biological Process: metabolic process; organelle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule nucleation; meiotic spindle organization and biogenesis; cytoplasmic microtubule organization and biogenesis Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4 |
| NCBI Summary: | This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009] |
| UniProt Code: | P23258 |
| NCBI GenInfo Identifier: | 20455518 |
| NCBI Gene ID: | 7283 |
| NCBI Accession: | P23258.2 |
| UniProt Secondary Accession: | P23258,Q53X79, Q9BW59, |
| UniProt Related Accession: | P23258 |
| Molecular Weight: | 451 |
| NCBI Full Name: | Tubulin gamma-1 chain |
| NCBI Synonym Full Names: | tubulin, gamma 1 |
| NCBI Official Symbol: | TUBG1 |
| NCBI Official Synonym Symbols: | TUBG; GCP-1; CDCBM4; TUBGCP1 |
| NCBI Protein Information: | tubulin gamma-1 chain; tubulin, gamma polypeptide; gamma-tubulin complex component 1 |
| UniProt Protein Name: | Tubulin gamma-1 chain |
| UniProt Synonym Protein Names: | Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1 |
| UniProt Gene Name: | TUBG1 |
| UniProt Entry Name: | TBG1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using gamma-Tubulin antibody (CAB14554) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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