Cell Biology Antibodies 5

Anti-GALNT6 Antibody (CAB14853)

(No reviews yet) Write a Review
SKU:
CAB14853
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GALNT6 Antibody
Antibody SKU:CAB14853
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-140 of human GALNT6 (NP_009141.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, BxPC-3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-140 of human GALNT6 (NP_009141.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DVSS REEA TEKP WLKS LVSR KDHV LDLM LEAM NNLR DSMP KLQI RAPE AQQT LFSI NQSC LPGF YTPA ELKP FWER PPQD PNAP GADG KAFQ KSKW TPLE TQEK EEGY KKH
Gene ID:11226
Uniprot:Q8NCL4
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:71kDa
Observed MW:71kDa
Synonyms:GALNT6, GALNAC-T6, GalNAcT6
Background:This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin.
UniProt Protein Function:GALNT6: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides.
UniProt Protein Details:

Protein type:Membrane protein, integral; EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Transferase

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi apparatus; Golgi membrane; perinuclear region of cytoplasm

Molecular Function:polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: O-glycan processing; protein amino acid O-linked glycosylation

NCBI Summary:This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin. [provided by RefSeq, Jul 2008]
UniProt Code:Q8NCL4
NCBI GenInfo Identifier:51316028
NCBI Gene ID:11226
NCBI Accession:Q8NCL4.2
UniProt Secondary Accession:Q8NCL4,Q8IYH4, Q9H6G2, Q9UIV5,
UniProt Related Accession:Q8NCL4
Molecular Weight:71,159 Da
NCBI Full Name:Polypeptide N-acetylgalactosaminyltransferase 6
NCBI Synonym Full Names:polypeptide N-acetylgalactosaminyltransferase 6
NCBI Official Symbol:GALNT6  
NCBI Official Synonym Symbols:GalNAcT6; GALNAC-T6  
NCBI Protein Information:polypeptide N-acetylgalactosaminyltransferase 6
UniProt Protein Name:Polypeptide N-acetylgalactosaminyltransferase 6
UniProt Synonym Protein Names:Polypeptide GalNAc transferase 6; GalNAc-T6; pp-GaNTase 6; Protein-UDP acetylgalactosaminyltransferase 6; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
Protein Family:Polypeptide N-acetylgalactosaminyltransferase
UniProt Gene Name:GALNT6  
UniProt Entry Name:GALT6_HUMAN
Anti-GALNT6 Antibody (CAB14853)Western blot analysis of extracts of various cell lines, using GALNT6 antibody (CAB14853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
View AllClose

0 Reviews

View AllClose

Related Products