Antibody Name:	Anti-GALNT2 Antibody
Antibody SKU:	CAB6910
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 442-571 of human GALNT2 (NP_004472.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, HepG2, mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 442-571 of human GALNT2 (NP_004472.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HQDI AFGA LQQG TNCL DTLG HFAD GVVG VYEC HNAG GNQE WALT KEKS VKHM DLCL TVVD RAPG SLIK LQGC REND SRQK WEQI EGNS KLRH VGSN LCLD SRTA KSGG LSVE VCGP ALSQ QWKF TLNL QQ
Gene ID:	2590
Uniprot:	Q10471
Cellular Location:	Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	30kDa/64kDa
Observed MW:	65kDa

Synonyms:

GALNT2, GalNAc-T2

Background:

This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GALNT2: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b.
UniProt Protein Details:	Protein type:Membrane protein, integral; EC 2.4.1.41; Transferase; Glycan Metabolism - O-glycan biosynthesis Chromosomal Location of Human Ortholog: 1q41-q42 Cellular Component: endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; Golgi stack; membrane; perinuclear region of cytoplasm Molecular Function:manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity; protein binding Biological Process: immunoglobulin biosynthetic process; O-glycan processing; protein amino acid O-linked glycosylation; protein amino acid O-linked glycosylation via serine; protein amino acid O-linked glycosylation via threonine
NCBI Summary:	This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	Q10471
NCBI GenInfo Identifier:	51315838
NCBI Gene ID:	2590
NCBI Accession:	Q10471.1
UniProt Secondary Accession:	Q10471,Q9NPY4, A8K1Y3, B7Z8V8, C5HU00,
UniProt Related Accession:	Q10471
Molecular Weight:	30,256 Da
NCBI Full Name:	Polypeptide N-acetylgalactosaminyltransferase 2
NCBI Synonym Full Names:	polypeptide N-acetylgalactosaminyltransferase 2
NCBI Official Symbol:	GALNT2
NCBI Official Synonym Symbols:	GalNAc-T2
NCBI Protein Information:	polypeptide N-acetylgalactosaminyltransferase 2
UniProt Protein Name:	Polypeptide N-acetylgalactosaminyltransferase 2
UniProt Synonym Protein Names:	Polypeptide GalNAc transferase 2; GalNAc-T2; pp-GaNTase 2
Protein Family:	Polypeptide N-acetylgalactosaminyltransferase
UniProt Gene Name:	GALNT2
UniProt Entry Name:	GALT2_HUMAN

	Western blot analysis of extracts of various cell lines, using GALNT2 antibody (CAB6910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using GALNT2 Antibody (CAB6910) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using GALNT2 Antibody (CAB6910) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using GALNT2 antibody (CAB6910) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using GALNT2 antibody (CAB6910) at dilution of 1:100. Blue: DAPI for nuclear staining.