Name: Anti-GADD45A Antibody (CAB17470)
Brand: Cell Cycle Antibodies 2
SKU: CAB17470
Availability: OutOfStock

Antibody Name:	Anti-GADD45A Antibody
Antibody SKU:	CAB17470
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of Human GADD45A.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat brain

Immunogen:	A synthetic peptide of Human GADD45A.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1647
Uniprot:	P24522
Cellular Location:	Nucleus
Calculated MW:	14kDa/18kDa
Observed MW:	18kDa

Western blot - GADD45A Rabbit pAb (CAB17470)

Synonyms:

DDIT1, GADD45, GADD45A

Background:

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

UniProt Protein Function:	GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p31.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity
NCBI Summary:	This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]
UniProt Code:	P24522
NCBI GenInfo Identifier:	120751
NCBI Gene ID:	1647
NCBI Accession:	P24522.1
UniProt Secondary Accession:	P24522,Q5TCA7, Q5TCA8,
UniProt Related Accession:	P24522
Molecular Weight:	14,622 Da
NCBI Full Name:	Growth arrest and DNA damage-inducible protein GADD45 alpha
NCBI Synonym Full Names:	growth arrest and DNA damage inducible alpha
NCBI Official Symbol:	GADD45A
NCBI Official Synonym Symbols:	DDIT1; GADD45
NCBI Protein Information:	growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Protein Name:	Growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Synonym Protein Names:	DNA damage-inducible transcript 1 protein; DDIT-1
Protein Family:	Growth arrest and DNA damage-inducible protein
UniProt Gene Name:	GADD45A
UniProt Entry Name:	GA45A_HUMAN