Cell Cycle Antibodies 1
Anti-GADD45A Antibody (CAB13486)
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- SKU:
- CAB13486
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-GADD45A Antibody |
| Antibody SKU: | CAB13486 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTLE EFSA GEQK TERM DKVG DALE EVLS KALS QRTI TVGV YEAA KLLN VDPD NVVL CLLA ADED DDRD VALQ IHFT LIQA FCCE NDIN ILRV SNPG RLAE LLLL ETDA GPAA SEGA EQPP DLHC VLVT NPHS SQWK DPAL SQLI CFCR ESRY MDQW VPVI NLPE R |
| Gene ID: | 1647 |
| Uniprot: | P24522 |
| Cellular Location: | Nucleus |
| Calculated MW: | 14kDa/18kDa |
| Observed MW: | 18kDa |
| Synonyms: | DDIT1, GADD45, GADD45A |
| Background: | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p31.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity |
| NCBI Summary: | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010] |
| UniProt Code: | P24522 |
| NCBI GenInfo Identifier: | 120751 |
| NCBI Gene ID: | 1647 |
| NCBI Accession: | P24522.1 |
| UniProt Secondary Accession: | P24522,Q5TCA7, Q5TCA8, |
| UniProt Related Accession: | P24522 |
| Molecular Weight: | 14,622 Da |
| NCBI Full Name: | Growth arrest and DNA damage-inducible protein GADD45 alpha |
| NCBI Synonym Full Names: | growth arrest and DNA damage inducible alpha |
| NCBI Official Symbol: | GADD45A |
| NCBI Official Synonym Symbols: | DDIT1; GADD45 |
| NCBI Protein Information: | growth arrest and DNA damage-inducible protein GADD45 alpha |
| UniProt Protein Name: | Growth arrest and DNA damage-inducible protein GADD45 alpha |
| UniProt Synonym Protein Names: | DNA damage-inducible transcript 1 protein; DDIT-1 |
| Protein Family: | Growth arrest and DNA damage-inducible protein |
| UniProt Gene Name: | GADD45A |
| UniProt Entry Name: | GA45A_HUMAN |
 | Western blot analysis of extracts of mouse liver, using GADD45A antibody (CAB13486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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