Neuroscience
Anti-GAD2 Antibody (CAB0971)
- SKU:
- CAB0971
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-GAD2 Antibody |
| Antibody SKU: | CAB0971 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD2 (NP_001127838.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD2 (NP_001127838.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PLQC SALL VREE GLMQ NCNQ MHAS YLFQ QDKH YDLS YDTG DKAL QCGR HVDV FKLW LMWR AKGT TGFE AHVD KCLE LAEY LYNI IKNR EGYE MVFD GKPQ HTNV CFWY IPPS LRTL EDNE ERMS RLSK VAPV IKAR MMEY GTTM VSYQ PLGD KVNF FRMV ISNP AATH QDID FLIE EIER LGQD L |
| Gene ID: | 2572 |
| Uniprot: | Q05329 |
| Cellular Location: | Cell junction, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Lipid-anchor, Peripheral membrane protein, cytosol, presynaptic cell membrane, synapse |
| Calculated MW: | 65kDa |
| Observed MW: | 70kDa |
| Synonyms: | GAD2, GAD65 |
| Background: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. |
| UniProt Protein Function: | GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 10p11.23 Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cytosol; cell junction Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process |
| NCBI Summary: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| UniProt Code: | Q05329 |
| NCBI GenInfo Identifier: | 1352216 |
| NCBI Gene ID: | 2572 |
| NCBI Accession: | Q05329.1 |
| UniProt Related Accession: | Q05329 |
| Molecular Weight: | |
| NCBI Full Name: | Glutamate decarboxylase 2 |
| NCBI Synonym Full Names: | glutamate decarboxylase 2 |
| NCBI Official Symbol: | GAD2 |
| NCBI Official Synonym Symbols: | GAD65 |
| NCBI Protein Information: | glutamate decarboxylase 2 |
| UniProt Protein Name: | Glutamate decarboxylase 2 |
| UniProt Synonym Protein Names: | 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform |
| Protein Family: | Glutamate decarboxylase |
| UniProt Gene Name: | GAD2 |
| UniProt Entry Name: | DCE2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GAD2 antibody (CAB0971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GAD2 Antibody (CAB0971) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat brain using GAD2 Rabbit pAb (CAB0971) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse brain using GAD2 Rabbit pAb (CAB0971) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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