Neuroscience
Anti-GAD1 Antibody (CAB2938)
- SKU:
- CAB2938
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-GAD1 Antibody |
| Antibody SKU: | CAB2938 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD1 (NP_000808.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, Jurkat, Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD1 (NP_000808.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASS TPSS SATS SNAG ADPN TTNL RPTT YDTW CGVA HGCT RKLG LKIC GFLQ RTNS LEEK SRLV SAFK ERQS SKNL LSCE NSDR DARF RRTE TDFS NLFA RDLL PAKN GEEQ TVQF LLEV VDIL LNYV RKTF DRST KVLD FHHP HQLL EGME GFNL ELSD HPES LEQI LVDC RDTL KYGV RTGH PRFF NQLS TGLD IIGL |
| Gene ID: | 2571 |
| Uniprot: | Q99259 |
| Cellular Location: | |
| Calculated MW: | 25kDa/47kDa/66kDa |
| Observed MW: | 67kDa |
| Synonyms: | GAD1, CPSQ1, GAD, SCP |
| Background: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. |
| UniProt Protein Function: | GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Lyase Chromosomal Location of Human Ortholog: 2q31 Cellular Component: plasma membrane; intracellular; vesicle membrane Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process Disease: Cerebral Palsy, Spastic Quadriplegic, 1 |
| NCBI Summary: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99259 |
| NCBI GenInfo Identifier: | 1352213 |
| NCBI Gene ID: | 2571 |
| NCBI Accession: | Q99259.1 |
| UniProt Secondary Accession: | Q99259,Q49AK1, Q53TQ7, Q9BU91, Q9UHH4, |
| UniProt Related Accession: | Q99259 |
| Molecular Weight: | 66,897 Da |
| NCBI Full Name: | Glutamate decarboxylase 1 |
| NCBI Synonym Full Names: | glutamate decarboxylase 1 (brain, 67kDa) |
| NCBI Official Symbol: | GAD1 |
| NCBI Official Synonym Symbols: | GAD; SCP; CPSQ1 |
| NCBI Protein Information: | glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform |
| UniProt Protein Name: | Glutamate decarboxylase 1 |
| UniProt Synonym Protein Names: | 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform |
| Protein Family: | Glutamate decarboxylase |
| UniProt Gene Name: | GAD1 |
| UniProt Entry Name: | DCE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GAD1 antibody (CAB2938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GAD1 Rabbit pAb (CAB2938) at dilution of 1:150 (40x lens). |
 | Immunofluorescence analysis of Rat eyeball using GAD1 antibody (CAB2938) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of Mouse eyeball using GAD1 antibody (CAB2938) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using GAD1 antibody (CAB2938) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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