Name: Anti-GABRA4 Antibody (CAB3870)
Brand: Signal Transduction Antibodies 2
SKU: CAB3870
Availability: OutOfStock

Antibody Name:	Anti-GABRA4 Antibody
Antibody SKU:	CAB3870
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 36-258 of human GABRA4 (NP_000800.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 36-258 of human GABRA4 (NP_000800.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QNQK EEKL CTEN FTRI LDSL LDGY DNRL RPGF GGPV TEVK TDIY VTSF GPVS DVEM EYTM DVFF RQTW IDKR LKYD GPIE ILRL NNMM VTKV WTPD TFFR NGKK SVSH NMTA PNKL FRIM RNGT ILYT MRLT ISAE CPMR LVDF PMDG HACP LKFG SYAY PKSE MIYT WTKG PEKS VEVP KESS SLVQ YDLI GQTV SSET IKSI TGEY IVMT VYFH LRRK MGY
Gene ID:	2557
Uniprot:	P48169
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	61kDa
Observed MW:	72kDa

Synonyms:

GABRA4

Background:

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.

UniProt Protein Function:	GABRA4: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily.
UniProt Protein Details:	Protein type:Transporter; Transporter, ion channel; Membrane protein, integral; Channel, chloride; Channel, ligand-gated; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 4p12 Cellular Component: cell junction; integral to plasma membrane; plasma membrane; postsynaptic membrane Molecular Function:benzodiazepine receptor activity; chloride channel activity; extracellular ligand-gated ion channel activity; GABA-A receptor activity Biological Process: central nervous system development; gamma-aminobutyric acid signaling pathway; synaptic transmission; transmembrane transport; transport
NCBI Summary:	Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011]
UniProt Code:	P48169
NCBI GenInfo Identifier:	206729865
NCBI Gene ID:	2557
NCBI Accession:	P48169.2
UniProt Secondary Accession:	P48169,Q8IYR7,
UniProt Related Accession:	P48169
Molecular Weight:	61,623 Da
NCBI Full Name:	Gamma-aminobutyric acid receptor subunit alpha-4
NCBI Synonym Full Names:	gamma-aminobutyric acid type A receptor alpha4 subunit
NCBI Official Symbol:	GABRA4
NCBI Protein Information:	gamma-aminobutyric acid receptor subunit alpha-4
UniProt Protein Name:	Gamma-aminobutyric acid receptor subunit alpha-4
UniProt Synonym Protein Names:	GABA(A) receptor subunit alpha-4
Protein Family:	Gamma-aminobutyric acid receptor
UniProt Gene Name:	GABRA4
UniProt Entry Name:	GBRA4_HUMAN

Western blot analysis of extracts of rat kidney, using GABRA4 antibody (CAB3870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.

Antibodies

GABRA4 Antibody (PACO00830)

GABRA4 Antibody (PACO09386)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)