Name: Anti-GABBR2 Antibody (CAB6594)
Brand: Cell Biology Antibodies 10
SKU: CAB6594
Availability: OutOfStock

Antibody Name:	Anti-GABBR2 Antibody
Antibody SKU:	CAB6594
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-310 of human GABBR2 (NP_005449.5).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-310 of human GABBR2 (NP_005449.5).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLMP LTKE VAKG SIGR GVLP AVEL AIEQ IRNE SLLR PYFL DLRL YDTE CDNA KGLK AFYD AIKY GPNH LMVF GGVC PSVT SIIA ESLQ GWNL VQLS FAAT TPVL ADKK KYPY FFRT VPSD NAVN PAIL KLLK HYQW KRVG TLTQ DVQR FSEV RNDL TGVL YGED IEIS DTES FSND PCTS VKKL KGND VRII LGQF DQNM AAKV FCCA YEEN MYGS KYQW IIPG WYEP SWWE QVHT EANS SRCL RKNL LAA
Gene ID:	9568
Uniprot:	O75899
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	105kDa
Observed MW:	122kDa

Synonyms:

GABBR2, GABABR2, GPR51, GPRC3B, HG20, HRIHFB2099

Background:

The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.

UniProt Protein Function:	GABBR2: Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high- voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with ATF4 via its C-terminal region. Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
UniProt Protein Details:	Protein type:Receptor, GPCR; GPCR, family 3; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 9q22.1-q22.3 Cellular Component: postsynaptic membrane; neuron projection; integral to plasma membrane; cytoplasm; plasma membrane; cell junction Molecular Function:protein binding; GABA-B receptor activity Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; negative regulation of adenylate cyclase activity; gamma-aminobutyric acid signaling pathway Disease: Tobacco Addiction, Susceptibility To
NCBI Summary:	The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010]
UniProt Code:	O75899
NCBI GenInfo Identifier:	12643641
NCBI Gene ID:	9568
NCBI Accession:	O75899.1
UniProt Secondary Accession:	O75899,O75974, O75975, Q5VXZ2, Q8WX04, Q9P1R2, Q9UNR1 Q9UNS9,
UniProt Related Accession:	O75899
Molecular Weight:	941
NCBI Full Name:	Gamma-aminobutyric acid type B receptor subunit 2
NCBI Synonym Full Names:	gamma-aminobutyric acid (GABA) B receptor, 2
NCBI Official Symbol:	GABBR2
NCBI Official Synonym Symbols:	HG20; GPR51; GPRC3B; GABABR2; HRIHFB2099
NCBI Protein Information:	gamma-aminobutyric acid type B receptor subunit 2; gb2; GABA-BR2; GABA-B-R2; GABA-B receptor 2; GABA-B receptor, R2 subunit; G protein-coupled receptor 51; G-protein coupled receptor 51; gamma-aminobutyric acid B receptor 2
UniProt Protein Name:	Gamma-aminobutyric acid type B receptor subunit 2
UniProt Synonym Protein Names:	G-protein coupled receptor 51; HG20
Protein Family:	Gamma-aminobutyric acid type B receptor
UniProt Gene Name:	GABBR2
UniProt Entry Name:	GABR2_HUMAN

Western blot analysis of extracts of MCF-7 cells, using GABBR2 antibody (CAB6594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.

Antibodies

GABBR2 Antibody (PACO05782)

GABBR2 Antibody (PACO09381)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)