Name: Anti-GABARAPL2 Antibody (CAB7782)
Brand: Autophagy Antibodies
SKU: CAB7782
Availability: OutOfStock

Antibody Name:	Anti-GABARAPL2 Antibody
Antibody SKU:	CAB7782
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKWM FKED HSLE HRCV ESAK IRAK YPDR VPVI VEKV SGSQ IVDI DKRK YLVP SDIT VAQF MWII RKRI QLPS EKAI FLFV DKTV PQSS LTMG QLYE KEKD EDGF LYVA YSGE NTFG F
Gene ID:	11345
Uniprot:	P60520
Cellular Location:	Cytoplasmic vesicle, Golgi apparatus, autophagosome
Calculated MW:	13kDa
Observed MW:	14kDa

Synonyms:	GABARAPL2, ATG8, ATG8C, GATE-16, GATE16, GEF-2, GEF2
Background:

UniProt Protein Function:	GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR.
UniProt Protein Details:	Protein type:Vesicle; Microtubule-binding; Ubiquitin-like modifier; Autophagy; Adaptor/scaffold Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: autophagic vacuole; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; intracellular Molecular Function:ATPase binding; beta-tubulin binding; GABA receptor binding; protein binding; SNARE binding; ubiquitin protein ligase binding Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; intra-Golgi vesicle-mediated transport; macroautophagy; mitochondrion degradation; positive regulation of ATPase activity
UniProt Code:	P60520
NCBI GenInfo Identifier:	44888808
NCBI Gene ID:	11345
NCBI Accession:	P60520.1
UniProt Secondary Accession:	P60520,O08765, Q6FG91, Q9DCP8, Q9UQF7,
UniProt Related Accession:	P60520
Molecular Weight:	13,667 Da
NCBI Full Name:	Gamma-aminobutyric acid receptor-associated protein-like 2
NCBI Synonym Full Names:	GABA type A receptor associated protein like 2
NCBI Official Symbol:	GABARAPL2
NCBI Official Synonym Symbols:	ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
NCBI Protein Information:	gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Protein Name:	Gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Synonym Protein Names:	GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; MAP1 light chain 3-related protein
Protein Family:	Gamma-aminobutyric acid receptor-associated protein
UniProt Gene Name:	GABARAPL2
UniProt Entry Name:	GBRL2_HUMAN

	Western blot analysis of extracts of mouse brain, using GABARAPL2 antibody (CAB7782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using GABARAPL2 antibody (CAB7782) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using GABARAPL2 antibody (CAB7782) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A549 cells using GABARAPL2 antibody (CAB7782). Blue: DAPI for nuclear staining.