Autophagy Antibodies
Anti-GABARAPL1 Antibody (CAB7790)
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- SKU:
- CAB7790
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-GABARAPL1 Antibody |
| Antibody SKU: | CAB7790 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL1 (NP_113600.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, Mouse liver, Mouse kidney, Mouse brain, Rat brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL1 (NP_113600.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKFQ YKED HPFE YRKK EGEK IRKK YPDR VPVI VEKA PKAR VPDL DKRK YLVP SDLT VGQF YFLI RKRI HLRP EDAL FFFV NNTI PPTS ATMG QLYE DNHE EDYF LYVA YSDE SVYG K |
| Gene ID: | 23710 |
| Uniprot: | Q9H0R8 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endoplasmic reticulum, Golgi apparatus, Lipid-anchor, autophagosome, cytoskeleton |
| Calculated MW: | 14kDa/17kDa |
| Observed MW: | 16kDa |
| Synonyms: | GABARAPL1, APG8-LIKE, APG8L, ATG8, ATG8B, ATG8L, GEC1 |
| Background: |
| UniProt Protein Function: | GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1. |
| UniProt Protein Details: | Protein type:Vesicle; Adaptor/scaffold; Microtubule-binding; Autophagy; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 12p13.2 Cellular Component: autophagic vacuole; cytoplasmic vesicle membrane; cytosol; dendrite cytoplasm; endoplasmic reticulum; Golgi apparatus; intracellular; microtubule; mitochondrion Molecular Function:beta-tubulin binding; GABA receptor binding; protein binding; Tat protein binding; ubiquitin protein ligase binding Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation |
| UniProt Code: | Q9H0R8 |
| NCBI GenInfo Identifier: | 13899219 |
| NCBI Gene ID: | 23710 |
| NCBI Accession: | NP_113600.1 |
| UniProt Secondary Accession: | Q9H0R8,Q6FIE6, B4E0Y7, |
| UniProt Related Accession: | Q9H0R8 |
| Molecular Weight: | 17,008 Da |
| NCBI Full Name: | gamma-aminobutyric acid receptor-associated protein-like 1 |
| NCBI Synonym Full Names: | GABA type A receptor associated protein like 1 |
| NCBI Official Symbol: | GABARAPL1 |
| NCBI Official Synonym Symbols: | ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE |
| NCBI Protein Information: | gamma-aminobutyric acid receptor-associated protein-like 1 |
| UniProt Protein Name: | Gamma-aminobutyric acid receptor-associated protein-like 1 |
| UniProt Synonym Protein Names: | Early estrogen-regulated protein; GABA(A) receptor-associated protein-like 1; Glandular epithelial cell protein 1; GEC-1 |
| Protein Family: | Gamma-aminobutyric acid receptor-associated protein |
| UniProt Gene Name: | GABARAPL1 |
| UniProt Entry Name: | GBRL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GABARAPL1 antibody (CAB7790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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