Cell Biology Antibodies 10
Anti-GAB1 Antibody (CAB6248)
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- SKU:
- CAB6248
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GAB1 Antibody |
| Antibody SKU: | CAB6248 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 485-724 of human GAB1 (NP_997006.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 485-724 of human GAB1 (NP_997006.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FGMQ VPPP AHMG FRSS PKTP PRRP VPVA DCEP PPVD RNLK PDRK GQSP KILR LKPH GLER TDSQ TIGD FATR RKVK PAPL EIKP LPEW EELQ APVR SPIT RSFA RDSS RFPM SPRP DSVH STTS SSDS HDSE ENYV PMNP NLSS EDPN LFGS NSLD GGSS PMIK PKGD KQVE YLDL DLDS GKST PPRK QKSS GSGS SVAD ERVD YVVV DQQK TLAL KSTR EAWT DGRQ STES ETPA KSVK |
| Gene ID: | 2549 |
| Uniprot: | Q13480 |
| Cellular Location: | |
| Calculated MW: | 76kDa/80kDa |
| Observed MW: | 110kDa |
| Synonyms: | GAB1, GRB2 |
| Background: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GAB1: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Belongs to the GAB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q31.21 Cellular Component: cytosol Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity Biological Process: activation of JNK activity; cell proliferation; epidermal growth factor receptor signaling pathway; epidermis development; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; insulin receptor signaling pathway; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; regulation of cell migration; response to oxidative stress |
| NCBI Summary: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | Q13480 |
| NCBI GenInfo Identifier: | 90180201 |
| NCBI Gene ID: | 2549 |
| NCBI Accession: | Q13480.2 |
| UniProt Secondary Accession: | Q13480,Q4W5G2, Q6P1W2, A8K152, |
| UniProt Related Accession: | Q13480 |
| Molecular Weight: | 80,005 Da |
| NCBI Full Name: | GRB2-associated-binding protein 1 |
| NCBI Synonym Full Names: | GRB2 associated binding protein 1 |
| NCBI Official Symbol: | GAB1 |
| NCBI Protein Information: | GRB2-associated-binding protein 1 |
| UniProt Protein Name: | GRB2-associated-binding protein 1 |
| UniProt Synonym Protein Names: | GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1 |
| Protein Family: | GPI transamidase component |
| UniProt Gene Name: | GAB1 |
| UniProt Entry Name: | GAB1_HUMAN |
 | Western blot analysis of extracts of SH-SY5Y cells, using GAB1 antibody (CAB6248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 80s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody (CAB6248) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody (CAB6248) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody (CAB6248) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody (CAB6248) at dilution of 1:100 (40x lens). |
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