Metabolism Antibodies 1

Anti-FZD6 Antibody (CAB10503)

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SKU:
CAB10503
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-FZD6 Antibody
Antibody SKU:CAB10503
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-201 of human FZD6 (NP_003497.2).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:mouse kidney, rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-201 of human FZD6 (NP_003497.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HSLF TCEP ITVP RCMK MAYN MTFF PNLM GHYD QSIA AVEM EHFL PLAN LECS PNIE TFLC KAFV PTCI EQIH VVPP CRKL CEKV YSDC KKLI DTFG IRWP EELE CDRL QYCD ETVP VTFD PHTE FLGP QKKT EQVQ RDIG FWCP RHLK TSGG QGYK FLGI DQCA PPCP NMYF KSDE LEFA KSF
Gene ID:8323
Uniprot:O60353
Cellular Location:Apical cell membrane, Cell membrane, Cell surface, Cytoplasmic vesicle membrane, Membrane, Multi-pass membrane protein
Calculated MW:75kDa/79kDa
Observed MW:79kDa
Synonyms:FZD6, FZ-6, FZ6, HFZ6, NDNC10
Background:This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.
UniProt Protein Function:FZD6: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney. Belongs to the G-protein coupled receptor Fz/Smo family.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 8q22.3-q23.1

Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function:G-protein coupled receptor activity; protein binding; ubiquitin protein ligase binding; Wnt receptor activity; Wnt-protein binding

Biological Process: negative regulation of transcription factor activity; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Nail Disorder, Nonsyndromic Congenital, 10

NCBI Summary:This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]
UniProt Code:O60353
NCBI GenInfo Identifier:215273986
NCBI Gene ID:8323
NCBI Accession:O60353.2
UniProt Secondary Accession:O60353,Q6N0A5, Q6P9C3, Q8WXR9, B4DRN0,
UniProt Related Accession:O60353
Molecular Weight:75,579 Da
NCBI Full Name:Frizzled-6
NCBI Synonym Full Names:frizzled class receptor 6
NCBI Official Symbol:FZD6  
NCBI Official Synonym Symbols:FZ6; FZ-6; HFZ6; NDNC10  
NCBI Protein Information:frizzled-6
UniProt Protein Name:Frizzled-6
Protein Family:Frizzled
UniProt Gene Name:FZD6  
UniProt Entry Name:FZD6_HUMAN
Anti-FZD6 Antibody (CAB10503)Western blot analysis of extracts of various cell line, using FZD6 antibody (CAB10503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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