Description
| Antibody Name: | Anti-FZD5 Antibody |
| Antibody SKU: | CAB12775 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-240 of human FZD5 (NP_003459.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-240 of human FZD5 (NP_003459.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RMSC DRLP VLGR DAEV LCMD YNRS EATT APPR PFPA KPTL PGPP GAPA SGGE CPAG GPFV CKCR EPFV PILK ESHP LYNK VRTG QVPN CAVP CYQP SFSA DERT FATF WIG |
| Gene ID: | 7855 |
| Uniprot: | Q13467 |
| Cellular Location: | Cell membrane, Golgi apparatus membrane, Multi-pass membrane protein |
| Calculated MW: | 64kDa |
| Observed MW: | 65kDa |
| Synonyms: | FZD5, C2orf31, HFZ5 |
| Background: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. |
| UniProt Protein Function: | FZD5: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway. Interacts with GOPC. Belongs to the G-protein coupled receptor Fz/Smo family. |
| UniProt Protein Details: | Protein type:GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 2q33.3 Cellular Component: cell surface; early endosome membrane; plasma membrane Molecular Function:beta-amyloid binding; protein binding; protein kinase binding; ubiquitin protein ligase binding; Wnt receptor activity; Wnt-protein binding Biological Process: determination of anterior/posterior axis, embryo; embryonic axis specification; neuron differentiation; positive regulation of interferon-gamma production; positive regulation of T cell cytokine production; positive regulation of transcription from RNA polymerase II promoter; Spemann organizer formation; synaptogenesis; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13467 |
| NCBI GenInfo Identifier: | 116242481 |
| NCBI Gene ID: | 7855 |
| NCBI Accession: | Q13467.2 |
| UniProt Secondary Accession: | Q13467,Q53R22, A8K2X1, B2RCZ1, |
| UniProt Related Accession: | Q13467 |
| Molecular Weight: | 65kDa |
| NCBI Full Name: | Frizzled-5 |
| NCBI Synonym Full Names: | frizzled class receptor 5 |
| NCBI Official Symbol: | FZD5 |
| NCBI Official Synonym Symbols: | HFZ5; C2orf31 |
| NCBI Protein Information: | frizzled-5 |
| UniProt Protein Name: | Frizzled-5 |
| UniProt Synonym Protein Names: | FzE5 |
| Protein Family: | Frizzled |
| UniProt Gene Name: | FZD5 |
 | Western blot analysis of extracts of rat kidney, using FZD5 antibody (CAB12775) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
Additional Information
Product Type: |
Antibody |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
Antibody Type: |
Polyclonal Antibody |
Research Area: |
Metabolism |
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