Neuroscience

Anti-FZD3 Antibody (CAB10063)

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SKU:
CAB10063
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-FZD3 Antibody
Antibody SKU:CAB10063
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 23-205 of human FZD3 (NP_059108.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HeLa, MCF7, Mouse brain, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 23-205 of human FZD3 (NP_059108.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HSLF SCEP ITLR MCQD LPYN TTFM PNLL NHYD QQTA ALAM EPFH PMVN LDCS RDFR PFLC ALYA PICM EYGR VTLP CRRL CQRA YSEC SKLM EMFG VPWP EDME CSRF PDCD EPYP RLVD LNLA GEPT EGAP VAVQ RDYG FWCP RELK IDPD LGYS FLHV RDCS PPCP NMYF RREE LSFA RYF
Gene ID:7976
Uniprot:Q9NPG1
Cellular Location:Apical cell membrane, Cell membrane, Cell surface, Membrane, Multi-pass membrane protein
Calculated MW:76kDa
Observed MW:76kDa
Synonyms:FZD3, Fz-3
Background:This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia.
UniProt Protein Function:FZD3: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Receptor, GPCR; GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function:G-protein coupled receptor activity; PDZ domain binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: negative regulation of mitotic cell cycle, embryonic; neuron differentiation; positive regulation of neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway

NCBI Summary:This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]
UniProt Code:Q9NPG1
NCBI GenInfo Identifier:17433071
NCBI Gene ID:7976
NCBI Accession:Q9NPG1.1
UniProt Secondary Accession:Q9NPG1,A8K615,
UniProt Related Accession:Q9NPG1
Molecular Weight:– Da
NCBI Full Name:Frizzled-3
NCBI Synonym Full Names:frizzled class receptor 3
NCBI Official Symbol:FZD3  
NCBI Official Synonym Symbols:Fz-3  
NCBI Protein Information:frizzled-3
UniProt Protein Name:Frizzled-3
UniProt Gene Name:FZD3  
UniProt Entry Name:FZD3_HUMAN
Anti-FZD3 Antibody (CAB10063)Western blot analysis of extracts of various cell lines, using FZD3 antibody (CAB10063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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