ChIP Antibodies
Anti-FZD10 Antibody (CAB15399)
- SKU:
- CAB15399
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-FZD10 Antibody |
| Antibody SKU: | CAB15399 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 135-230 of human FZD10 (NP_009128.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | A-549, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 135-230 of human FZD10 (NP_009128.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RKLP NKND PNYL CMEA PNNG SDEP TRGS GLFP PLFR PQRP HSAQ EHPL KDGG PGRG GCDN PGKF HHVE KSAS CAPL CTPG VDVY WSRE DKRF AVVW |
| Gene ID: | 11211 |
| Uniprot: | Q9ULW2 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 65kDa |
| Observed MW: | 72kDa |
| Synonyms: | FZD10, CD350, FZ-10, Fz10, FzE7, hFz10 |
| Background: | This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. |
| UniProt Protein Function: | FZD10: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; GPCR, Fz/Smo family; Receptor, GPCR Chromosomal Location of Human Ortholog: 12q24.33 Cellular Component: cell surface; cytoplasm; integral to plasma membrane Molecular Function:G-protein coupled receptor activity; protein binding; Wnt-protein binding Biological Process: neuron differentiation; positive regulation of GTPase activity; positive regulation of JNK activity; regulation of actin cytoskeleton organization and biogenesis; Wnt receptor signaling pathway through beta-catenin |
| NCBI Summary: | This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9ULW2 |
| NCBI GenInfo Identifier: | 17433091 |
| NCBI Gene ID: | 11211 |
| NCBI Accession: | Q9ULW2.1 |
| UniProt Related Accession: | Q9ULW2 |
| Molecular Weight: | 65,336 Da |
| NCBI Full Name: | Frizzled-10 |
| NCBI Synonym Full Names: | frizzled class receptor 10 |
| NCBI Official Symbol: | FZD10 |
| NCBI Official Synonym Symbols: | Fz10; FzE7; CD350; FZ-10; hFz10 |
| NCBI Protein Information: | frizzled-10 |
| UniProt Protein Name: | Frizzled-10 |
| UniProt Synonym Protein Names: | FzE7; CD_antigen: CD350 |
| Protein Family: | Frizzled |
| UniProt Gene Name: | FZD10 |
| UniProt Entry Name: | FZD10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FZD10 antibody (CAB15399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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