Name: Anti-FXYD1 Antibody (CAB15082)
Brand: Signal Transduction Antibodies 2
SKU: CAB15082
Availability: OutOfStock

Antibody Name:	Anti-FXYD1 Antibody
Antibody SKU:	CAB15082
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-92 of human FXYD1 (NP_005022.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	mouse brain, mouse heart, mouse liver, rat heart, rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-92 of human FXYD1 (NP_005022.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASL GHIL VFCV GLLT MAKA ESPK EHDP FTYD YQSL QIGG LVIA GILF ILGI LIVL SRRC RCKF NQQQ RTGE PDEE EGTF RSSI RRLS TRRR
Gene ID:	5348
Uniprot:	O00168
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	10kDa
Observed MW:	16kDa

Synonyms:

FXYD1, PLM

Background:

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na, K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature.

UniProt Protein Function:	PLM: a protein of the FXYD family of small ion transport regulators or channels. Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May have a functional role in muscle contraction. Major plasma membrane substrate for PKA and PKC in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation.Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining.
UniProt Protein Details:	Protein type:Channel, misc.; Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: integral to plasma membrane; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:sodium channel regulator activity Biological Process: chloride transport; muscle contraction; regulation of heart contraction
NCBI Summary:	This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. [provided by RefSeq, Jul 2008]
UniProt Code:	O00168
NCBI GenInfo Identifier:	22654269
NCBI Gene ID:	5348
NCBI Accession:	O00168.2
UniProt Secondary Accession:	O00168,A8K196,
UniProt Related Accession:	O00168
Molecular Weight:	10,441 Da
NCBI Full Name:	Phospholemman
NCBI Synonym Full Names:	FXYD domain containing ion transport regulator 1
NCBI Official Symbol:	FXYD1
NCBI Official Synonym Symbols:	PLM
NCBI Protein Information:	phospholemman
UniProt Protein Name:	Phospholemman
UniProt Synonym Protein Names:	FXYD domain-containing ion transport regulator 1
Protein Family:	Phospholemman
UniProt Gene Name:	FXYD1
UniProt Entry Name:	PLM_HUMAN

Western blot analysis of extracts of various cell lines, using FXYD1 antibody (CAB15082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90.

Antibodies

FXYD1 Antibody (PACO09367)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)