Cell Biology Antibodies 7
Anti-FUT4 Antibody (CAB16320)
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- SKU:
- CAB16320
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-FUT4 Antibody |
| Antibody SKU: | CAB16320 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human FUT4 (NP_002024.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human FUT4 (NP_002024.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GRDS APRP PPDC RLRF NISG CRLL TDRA SYGE AQAV LFHH RDLV KGPP DWPP PWGI QAHT AEEV DLRV LDYE EAAA AAEA LATS SPRP PGQR WVWM NFES P |
| Gene ID: | 2526 |
| Uniprot: | P22083 |
| Cellular Location: | Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein |
| Calculated MW: | 59kDa |
| Observed MW: | 59KDa |
| Synonyms: | FUT4, CD15, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1 |
| Background: | The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). |
| UniProt Protein Function: | FUT4: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Belongs to the glycosyltransferase 10 family. |
| UniProt Protein Details: | Protein type:Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.-; Transferase; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q21 Cellular Component: Golgi apparatus; membrane Molecular Function:alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity Biological Process: carbohydrate metabolic process; protein amino acid glycosylation |
| NCBI Summary: | The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009] |
| UniProt Code: | P22083 |
| NCBI GenInfo Identifier: | 226694189 |
| NCBI Gene ID: | 2526 |
| NCBI Accession: | P22083.3 |
| UniProt Secondary Accession: | P22083,B2RMS0, |
| UniProt Related Accession: | P22083 |
| Molecular Weight: | 59,084 Da |
| NCBI Full Name: | Alpha-(1,3)-fucosyltransferase 4 |
| NCBI Synonym Full Names: | fucosyltransferase 4 |
| NCBI Official Symbol: | FUT4 |
| NCBI Official Synonym Symbols: | LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV |
| NCBI Protein Information: | alpha-(1,3)-fucosyltransferase 4 |
| UniProt Protein Name: | Alpha-(1,3)-fucosyltransferase 4 |
| UniProt Synonym Protein Names: | ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase |
| Protein Family: | Probable fucosyltransferase |
| UniProt Gene Name: | FUT4 |
| UniProt Entry Name: | FUT4_HUMAN |
 | Western blot analysis of extracts of HL-60 cells, using FUT4 Polyclonal Antibody (CAB16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded human colon using FUT4 Rabbit pAb (CAB16320) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using FUT4 Rabbit pAb (CAB16320) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of human colon carcinoma using FUT4 Rabbit pAb (CAB16320) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of human colon carcinoma using FUT4 Rabbit pAb (CAB16320) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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