Cell Biology Antibodies 5

Anti-FUT3 Antibody (CAB15058)

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SKU:
CAB15058
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FUT3 Antibody
Antibody SKU:CAB15058
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FUT3 (NP_000140.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Rat
Positive Samples:A-549, HT-29, 293T, LO2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FUT3 (NP_000140.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDPL GAAK PQWP WRRC LAAL LFQL LVAV CFFS YLRV SRDD ATGS PRAP SGSS RQDT TPTR PTLL ILLW TWPF HIPV ALSR CSEM VPGT ADCH ITAD RKVY
Gene ID:2525
Uniprot:P21217
Cellular Location:Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein
Calculated MW:42kDa
Observed MW:50kDa
Synonyms:FUT3, CD174, FT3B, FucT-III, LE, Les
Background:The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1, 3)-fucosyltransferase and alpha(1, 4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
UniProt Protein Function:FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; EC 2.4.1.65

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; membrane

Molecular Function:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: biopolymer glycosylation; cell-cell recognition; oligosaccharide biosynthetic process

NCBI Summary:The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P21217
NCBI GenInfo Identifier:121137
NCBI Gene ID:2525
NCBI Accession:P21217.1
UniProt Secondary Accession:P21217,Q32NE7, Q99448, Q99449, B5U7U9, B5U7V0,
UniProt Related Accession:P21217
Molecular Weight:42,117 Da
NCBI Full Name:Galactoside 3(4)-L-fucosyltransferase
NCBI Synonym Full Names:fucosyltransferase 3 (Lewis blood group)
NCBI Official Symbol:FUT3  
NCBI Official Synonym Symbols:LE; Les; FT3B; CD174; FucT-III  
NCBI Protein Information:galactoside 3(4)-L-fucosyltransferase
UniProt Protein Name:Galactoside 3(4)-L-fucosyltransferase
UniProt Synonym Protein Names:Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III
Protein Family:Fucosyltransferase
UniProt Gene Name:FUT3  
UniProt Entry Name:FUT3_HUMAN
Anti-FUT3 Antibody (CAB15058)Western blot analysis of extracts of various cell lines, using FUT3 antibody (CAB15058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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