Antibody Name:	Anti-FUS Antibody [KO Validated]
Antibody SKU:	CAB5921
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-937, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TIES VADY FKQI GIIK TNKK TGQP MINL YTDR ETGK LKGE ATVS FDDP PSAK AAID WFDG KEFS GNPI KVSF ATRR ADFN RGGG NGRG GRGR GGPM GRGG YGGG GSGG GGRG GFPS GGGG GGGQ QRAG DWKC PNPT CENM NFSW RNEC NQCK APKP DGPG GGPG GSHM GGNY GDDR RGGR GGYD RGGY RGRG GDRG GFRG GRGG GDRG GFGP GKMD SRGE HRQD RRER PY
Gene ID:	2521
Uniprot:	P35637
Cellular Location:	Nucleus
Calculated MW:	53kDa
Observed MW:	70kDa

Synonyms:

FUS, ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS

Background:

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

UniProt Protein Function:	hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; RNA-binding; Nuclear receptor co-regulator; DNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4
NCBI Summary:	This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]
UniProt Code:	P35637
NCBI GenInfo Identifier:	544357
NCBI Gene ID:	2521
NCBI Accession:	P35637.1
UniProt Secondary Accession:	P35637,Q9H4A8,
UniProt Related Accession:	P35637
Molecular Weight:	Calculated MW: 53kDaObserved MW: 70kDa
NCBI Full Name:	RNA-binding protein FUS
NCBI Synonym Full Names:	FUS RNA binding protein
NCBI Official Symbol:	FUS
NCBI Official Synonym Symbols:	TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
NCBI Protein Information:	RNA-binding protein FUS; oncogene FUS; oncogene TLS; fus-like protein; fused in sarcoma; 75 kDa DNA-pairing protein; fusion gene in myxoid liposarcoma; translocated in liposarcoma protein; heterogeneous nuclear ribonucleoprotein P2
UniProt Protein Name:	RNA-binding protein FUS
UniProt Synonym Protein Names:	75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein
Protein Family:	Fusion protein
UniProt Gene Name:	FUS
UniProt Entry Name:	FUS_HUMAN

	Western blot analysis of extracts of various cell lines, using FUS antibody (CAB5921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody (CAB5921) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using FUS antibody (CAB5921) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using FUS antibody (CAB5921) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using FUS antibody (CAB5921) at dilution of 1:100 (40x lens).