Metabolism Antibodies 3
Anti-Frizzled-9 Antibody (CAB8311)
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- SKU:
- CAB8311
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | FZD9 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8311 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 469-591 of human FZD9 (NP_003499.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | TE-1, 293T, HepG2, THP-1, U-251MG, Mouse heart, Mouse brain, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 469-591 of human FZD9 (NP_003499.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ERLN MDFW RLRA TEQP CAAA AGPG GRRD CSLP GGSV PTVA VFML KIFM SLVV GITS GVWV WSSK TFQT WQSL CYRK IAAG RARA KACR APGS YGRG THCH YKAP TVVL HMTK TDPS LENP THL |
| Gene ID: | 8326 |
| Uniprot: | O00144 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 64kDa |
| Observed MW: | 80kDa |
| Synonyms: | FZD9, CD349, FZD3 |
| Background: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. |
| UniProt Protein Function: | FZD9: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. |
| UniProt Protein Details: | Protein type:GPCR, Fz/Smo family; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cell surface; cytoplasm; plasma membrane Molecular Function:G-protein coupled receptor activity; protein heterodimerization activity; protein homodimerization activity; Wnt-protein binding Biological Process: nervous system development; Wnt receptor signaling pathway through beta-catenin |
| NCBI Summary: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00144 |
| NCBI GenInfo Identifier: | 17432964 |
| NCBI Gene ID: | 8326 |
| NCBI Accession: | O00144.1 |
| UniProt Related Accession: | O00144 |
| Molecular Weight: | 64,466 Da |
| NCBI Full Name: | Frizzled-9 |
| NCBI Synonym Full Names: | frizzled class receptor 9 |
| NCBI Official Symbol: | FZD9 |
| NCBI Official Synonym Symbols: | FZD3; CD349 |
| NCBI Protein Information: | frizzled-9 |
| UniProt Protein Name: | Frizzled-9 |
| UniProt Synonym Protein Names: | FzE6; CD_antigen: CD349 |
| Protein Family: | Frizzled |
| UniProt Gene Name: | FZD9 |
| UniProt Entry Name: | FZD9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FZD9 antibody (CAB8311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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