Cell Biology Antibodies 6

Anti-FREM2 Antibody (CAB15980)

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SKU:
CAB15980
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FREM2 Antibody
Antibody SKU:CAB15980
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse,
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 47-300 of human FREM2 (NP_997244.4).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse,
Positive Samples:Jurkat, HepG2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 47-300 of human FREM2 (NP_997244.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QPAA FGRA LLSP GLAG AAGV PAEE AIVL ANRG LRVP FGRE VWLD PLHD LVLQ VQPG DRCA VSVL DNDA LAQR PGRL SPKR FPCD FGPG EVRY SHLG ARSP SRDR VRLQ LRYD APGG AVVL PLVL EVEV VFTQ LEVV TRNL PLVV EELL GTSN ALDA RSLE FAFQ PETE ECRV GILS GLGA LPRY GELL HYPQ VPGG AREG GAPE TLLM DCKA FQEL GVRY RHTA ASRS PNRD WIPM VVEL RSRG APVG SPAL KR
Gene ID:341640
Uniprot:Q5SZK8
Cellular Location:Cell membrane, Extracellular side, Single-pass type I membrane protein
Calculated MW:295kDa/351kDa
Observed MW:351kDa
Synonyms:FREM2
Background:This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome.
UniProt Protein Function:FREM2: Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion. Defects in FREM2 are a cause of Fraser syndrome (FRASS). Fraser syndrome is a multisystem malformation usually comprising cryptophthalmos, cutaneous syndactyly, ear abnormalities, renal agenesis and congenital heart defects. Belongs to the FRAS1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Extracellular matrix

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: plasma membrane; integral to membrane; basement membrane

Molecular Function:calcium ion binding

Biological Process: morphogenesis of an epithelium; cell communication; homophilic cell adhesion; inner ear development

Disease: Fraser Syndrome

NCBI Summary:This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014]
UniProt Code:Q5SZK8
NCBI GenInfo Identifier:79749430
NCBI Gene ID:341640
NCBI Accession:NP_997244
UniProt Secondary Accession:Q5SZK8,Q4QQG1, Q5H9N8, Q5T6Q1, Q6N057, Q6ZSB4, Q7Z305 Q7Z341,
UniProt Related Accession:Q5SZK8
Molecular Weight:295,845 Da
NCBI Full Name:FRAS1-related extracellular matrix protein 2
NCBI Synonym Full Names:FRAS1 related extracellular matrix protein 2
NCBI Official Symbol:FREM2  
NCBI Protein Information:FRAS1-related extracellular matrix protein 2; ECM3 homolog
UniProt Protein Name:FRAS1-related extracellular matrix protein 2
UniProt Synonym Protein Names:ECM3 homolog
Protein Family:FRAS1-related extracellular matrix protein
UniProt Gene Name:FREM2  
UniProt Entry Name:FREM2_HUMAN
Anti-FREM2 Antibody (CAB15980)Western blot analysis of extracts of various cell lines, using FREM2 antibody (CAB15980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 150s.
Anti-FREM2 Antibody (CAB15980)Immunofluorescence analysis of NIH/3T3 cells using FREM2 Rabbit pAb (CAB15980) at dilution of 1:100. Blue: DAPI for nuclear staining.
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