Antibody Name:	Anti-FoxO3a Antibody
Antibody SKU:	CAB0102
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, 293T, PC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALPP SQPS PPGG LMQR GSSF PYTA KSSG LGSP TGSF NSTV FGPS SLNS LRQS PMQT IQEN RPAT FSSV SHYG NQTL QDLL ASDS LSHS DVMM TQSD PLMS QAST AVSA QNAR RNVM LRND PMMS FAAQ PTQG SLVN QNLL HHQH QTQG ALGG SRAL SNSV SNMG LSDS SSLG SAKH QQQS PASQ SMQT LSDS LSGS SLYS ASAN LPVM GHDK FPSD LDLD MFNG SLEC DMES IIRS ELMD ADGL DFNF DSLI STQN VVGL NVGN FTGA KQAS SQSW VPG
Gene ID:	56484
Uniprot:	Q9WVH4
Cellular Location:	Cytoplasm, Nucleus, cytosol
Calculated MW:	71kDa
Observed MW:	80-90KDa

Synonyms:

FOXO3, AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A

Background:

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

UniProt Protein Function:	FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.
UniProt Protein Details:	Protein type:Autophagy; DNA-binding; Oncoprotein; Transcription factor Chromosomal Location of Human Ortholog: 10 B2\|10 22.79 cM Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; nucleus Molecular Function:beta-catenin binding; chromatin DNA binding; DNA binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity Biological Process: aging; antral ovarian follicle growth; brain morphogenesis; DNA damage response, signal transduction by p53 class mediator; glucose homeostasis; initiation of primordial ovarian follicle growth; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; oocyte maturation; ovulation from ovarian follicle; positive regulation of apoptosis; positive regulation of erythrocyte differentiation; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of translation; tumor necrosis factor-mediated signaling pathway
UniProt Code:	Q9WVH4
NCBI GenInfo Identifier:	81917900
NCBI Gene ID:	56484
NCBI Accession:	Q9WVH4.1
UniProt Secondary Accession:	Q9WVH4,Q05CZ4, D3Z6Y6,
UniProt Related Accession:	Q9WVH4
Molecular Weight:	71,064 Da
NCBI Full Name:	Forkhead box protein O3
NCBI Synonym Full Names:	forkhead box O3
NCBI Official Symbol:	Foxo3
NCBI Official Synonym Symbols:	Fkhr2; C76856; FKHRL1; Foxo3a; 1110048B16Rik; 2010203A17Rik
NCBI Protein Information:	forkhead box protein O3
UniProt Protein Name:	Forkhead box protein O3
Protein Family:	Forkhead box protein
UniProt Gene Name:	Foxo3

	Western blot analysis of extracts of various cell lines, using FoxO3a antibody (CAB0102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded Human colon using FoxO3a Rabbit pAb (CAB0102) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse kidney using FoxO3a Rabbit pAb (CAB0102) at dilution of 1:50 (40x lens).
	Immunofluorescence analysis of C6 cells using FoxO3a Rabbit pAb (CAB0102) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using FoxO3a Rabbit pAb (CAB0102) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.