Name: Anti-FOXL2 Antibody (CAB16244)
Brand: Developmental Biology
SKU: CAB16244
Availability: OutOfStock

Antibody Name:	Anti-FOXL2 Antibody
Antibody SKU:	CAB16244
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human FOXL2 (NP_075555.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	OVCAR3, K-562, HeLa

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human FOXL2 (NP_075555.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HAAA APPP APPH HGAA APPP GQLS PASP ATAA PPAP APTS APGL QFAC ARQP ELAM MHCS YWDH DSKT GALH SRLD L
Gene ID:	668
Uniprot:	P58012
Cellular Location:	Nucleus
Calculated MW:	38kDa
Observed MW:	49kDa

Synonyms:	FOXL2, BPES, BPES1, PFRK, PINTO, POF3
Background:	This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3.

UniProt Protein Function:	FOXL2: Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans- differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Is a regulator of CYP19 expression. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES); also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II. Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 3q23 Cellular Component: nucleus Molecular Function:protein binding; DNA binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; caspase regulator activity; estrogen receptor binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; embryonic eye morphogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; female somatic sex determination; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; ovarian follicle development; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; menstruation; cell differentiation; negative regulation of transcription, DNA-dependent; extraocular skeletal muscle development; granulosa cell differentiation Disease: Blepharophimosis, Ptosis, And Epicanthus Inversus; Premature Ovarian Failure 3
NCBI Summary:	This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jun 2009]
UniProt Code:	P58012
NCBI GenInfo Identifier:	13626838
NCBI Gene ID:	668
NCBI Accession:	P58012.1
UniProt Secondary Accession:	P58012,Q4ZGJ3,
UniProt Related Accession:	P58012
Molecular Weight:	376
NCBI Full Name:	Forkhead box protein L2
NCBI Synonym Full Names:	forkhead box L2
NCBI Official Symbol:	FOXL2
NCBI Official Synonym Symbols:	BPES; PFRK; POF3; BPES1; PINTO
NCBI Protein Information:	forkhead box protein L2; forkhead transcription factor FOXL2
UniProt Protein Name:	Forkhead box protein L2
Protein Family:	Forkhead box protein
UniProt Gene Name:	FOXL2
UniProt Entry Name:	FOXL2_HUMAN

	Western blot analysis of extracts of various cell lines, using FOXL2 antibody (CAB16244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of rat ovary using FOXL2 Rabbit pAb (CAB16244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse ovary using FOXL2 Rabbit pAb (CAB16244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.