Name: Anti-FOSL2 Antibody (CAB2729)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB2729
Availability: OutOfStock

Antibody Name:	Anti-FOSL2 Antibody
Antibody SKU:	CAB2729
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 187-326 of human FOSL2 (NP_005244.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	BT-474, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 187-326 of human FOSL2 (NP_005244.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HGPV CKIS PEER RSPP APGL QPMR SGGG SVGA VVVK QEPL EEDS PSSS SAGL DKAQ RSVI KPIS IAGG FYGE EPLH TPIV VTST PAVT PGTS NLVF TYPS VLEQ ESPA SPSE SCSK AHRR SSSS GDQS SDSL NSPT LLAL
Gene ID:	2355
Uniprot:	P15408
Cellular Location:	Nucleus
Calculated MW:	31kDa/34kDa/35kDa
Observed MW:	35kDa

Synonyms:	FOSL2, FRA2
Background:	The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

UniProt Protein Function:	FRA2: an oncogenic transcription factor of the bZIP family, Fos subfamily. The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity. Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate but short-lived, while FRA1 and FRA2 expression persists longer. FRA2 and JUND up-regulate the expression of CCR4, MYB, MDM2, and BCL6, and are expressed at high levels in CCR4-expressing mature T-cell malignancies such as adult T-cell leukemia/lymphoma (ATLL) and cutaneous T-cell lymphomas (CTCLs). May play a role in regulating the transcription of ICOS downstream of TCR/CD28 and cytokine receptor signaling. Its transcription is significantly increased in metastatic clear cell renal cell carcinomas. Belongs to the bZIP family, Fos subfamily. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: cell death; regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq, Jul 2014]
UniProt Code:	P15408
NCBI GenInfo Identifier:	120487
NCBI Gene ID:	2355
NCBI Accession:	P15408.1
UniProt Secondary Accession:	P15408,Q6FG46, B2RD58, B3KP27, B4DYV4,
UniProt Related Accession:	P15408
Molecular Weight:	31,095 Da
NCBI Full Name:	Fos-related antigen 2
NCBI Synonym Full Names:	FOS like 2, AP-1 transcription factor subunit
NCBI Official Symbol:	FOSL2
NCBI Official Synonym Symbols:	FRA2
NCBI Protein Information:	fos-related antigen 2
UniProt Protein Name:	Fos-related antigen 2
Protein Family:	Fos-related antigen
UniProt Gene Name:	FOSL2
UniProt Entry Name:	FOSL2_HUMAN

	Western blot analysis of extracts of various cell lines, using FOSL2 antibody (CAB2729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human esophagus using FOSL2 antibody (CAB2729) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using FOSL2 antibody (CAB2729) at dilution of 1:100. Blue: DAPI for nuclear staining.