Antibody Name:	Anti-FOLR1 Antibody
Antibody SKU:	CAB15672
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-93 of human FOLR1 (NP_000793.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-93 of human FOLR1 (NP_000793.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RIAW ARTE LLNV CMNA KHHK EKPG PEDK LHEQ CRPW RKNA CCST NTSQ EAHK DVSY LYRF NWNH CGEM A
Gene ID:	2348
Uniprot:	P15328
Cellular Location:	Apical cell membrane, Cell membrane, Cytoplasmic vesicle, Endosome, GPI-anchor, Lipid-anchor, Secreted, clathrin-coated vesicle
Calculated MW:	29kDa
Observed MW:	37kDa

Synonyms:

FOLR1, FBP, FOLR

Background:

The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	FOLR1: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain- specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. Belongs to the folate receptor family.
UniProt Protein Details:	Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 11q13.3-q14.1 Cellular Component: anchored to external side of plasma membrane; apical plasma membrane; basolateral plasma membrane; cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi membrane; integral to plasma membrane; membrane; nucleus Molecular Function:drug binding; folic acid binding; folic acid transporter activity; methotrexate binding; receptor activity Biological Process: axon regeneration; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; folic acid transport; heart looping; receptor-mediated endocytosis; regulation of transforming growth factor beta receptor signaling pathway Disease: Neurodegeneration Due To Cerebral Folate Transport Deficiency
NCBI Summary:	The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	P15328
NCBI GenInfo Identifier:	544337
NCBI Gene ID:	2348
NCBI Accession:	P15328.3
UniProt Secondary Accession:	P15328,Q53EW2, Q6FGT8, Q6LC90, Q9UCT2,
UniProt Related Accession:	P15328
Molecular Weight:	29,819 Da
NCBI Full Name:	Folate receptor alpha
NCBI Synonym Full Names:	folate receptor 1
NCBI Official Symbol:	FOLR1
NCBI Official Synonym Symbols:	FBP; FOLR
NCBI Protein Information:	folate receptor alpha
UniProt Protein Name:	Folate receptor alpha
UniProt Synonym Protein Names:	Adult folate-binding protein; FBP; Folate receptor 1; Folate receptor, adult; KB cells FBP; Ovarian tumor-associated antigen MOv18
Protein Family:	Folate receptor
UniProt Gene Name:	FOLR1
UniProt Entry Name:	FOLR1_HUMAN

	Western blot analysis of extracts of Rat brain, using FOLR1 antibody (CAB15672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat testis using FOLR1 Rabbit pAb (CAB15672) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using FOLR1 Rabbit pAb (CAB15672) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using FOLR1 Rabbit pAb (CAB15672) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using FOLR1 Rabbit pAb (CAB15672) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.