Cell Biology Antibodies 1

Anti-FNBP1 Antibody (CAB10289)

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SKU:
CAB10289
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FNBP1 Antibody
Antibody SKU:CAB10289
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human FNBP1 (NP_055848.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SGC-7901, LO2, HeLa, U-87MG, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human FNBP1 (NP_055848.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSWG TELW DQFD NLEK HTQW GIDI LEKY IKFV KERT EIEL SYAK QLRN LSKK YQPK KNSK EEEE YKYT SCKA FISN LNEM NDYA GQHE VISE NMAS QIIV DLAR YVQE LKQE RKSN FHDG RKAQ QHIE TCWK QLES SKRR FERD CKEA DRAQ QYFE KMDA DINV TKAD VEKA RQQA QIRH QMAE DSKA DYSS ILQK FNHE QHEY YHTH IP
Gene ID:23048
Uniprot:Q96RU3
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Lysosome, Membrane, Peripheral membrane protein, cell cortex, clathrin-coated pit, cytoskeleton
Calculated MW:64kDa/68kDa/70kDa/71kDa
Observed MW:71kDa
Synonyms:FNBP1, FBP17
Background:The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain, a proline-rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBD), and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human.
UniProt Protein Function:FNBP1: May act as a link between RND2 signaling and regulation of the actin cytoskeleton. Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during the late stage of clathrin-mediated endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May be required for the lysosomal retention of FASLG/FASL. A chromosomal aberration involving FNBP1 is found in acute leukemias. Translocation t(9;11)(q34;q23) with MLL. The relatively low incidence of the MLL-FNBP1 fusion protein in acute leukemia may reflect the marginal capacity of this fusion protein to induce cellular transformation. Belongs to the FNBP1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Actin-binding; Vesicle

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytosol

Molecular Function:identical protein binding; protein binding

NCBI Summary:The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain, a proline-rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBD), and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. [provided by RefSeq, Jul 2008]
UniProt Code:Q96RU3
NCBI GenInfo Identifier:118572321
NCBI Gene ID:23048
NCBI Accession:Q96RU3.2
UniProt Secondary Accession:Q96RU3,O60301, Q3MIN8, Q5TC87, Q5TC88, Q6P658, Q7LGG2 Q9H8H8, Q9NWD1, B7ZL12, E9PGQ4,
UniProt Related Accession:Q96RU3
Molecular Weight:71kDa
NCBI Full Name:Formin-binding protein 1
NCBI Synonym Full Names:formin binding protein 1
NCBI Official Symbol:FNBP1  
NCBI Official Synonym Symbols:FBP17  
NCBI Protein Information:formin-binding protein 1
UniProt Protein Name:Formin-binding protein 1
UniProt Synonym Protein Names:Formin-binding protein 17; hFBP17
Protein Family:Formin-binding protein
UniProt Gene Name:FNBP1  
Anti-FNBP1 Antibody (CAB10289)Western blot analysis of extracts of various cell lines, using FNBP1 antibody (CAB10289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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