Antibody Name:	Anti-FMO5 Antibody
Antibody SKU:	CAB7673
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human FMO5 (NP_001138302.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human FMO5 (NP_001138302.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTKK RIAV IGGG VSGL SSIK CCVE EGLE PVCF ERTD DIGG LWRF QENP EEGR ASIY KSVI INTS KEMM CFSD YPIP DHYP NFMH NAQV LEYF RMYA KEFD LLKY IRFK TTVC SVKK QPDF ATSG QWEV VTES EGKK EMNV FDGV MVCT GHHT NAHL PLES FPGI EKFK GQYF HSRD YKNP EGFT GKRV IIIG IGNS GGDL AVEI SQTA KQVF LSTR RGAW ILNR VGDY GYPA DVLF SSRL THFI WKIC GQSL ANKY LEKK INQR FDHE MFGL KPKH RSKD IALT E
Gene ID:	2330
Uniprot:	P49326
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane
Calculated MW:	32kDa/52kDa/60kDa
Observed MW:	60kDa

Synonyms:

FMO5

Background:

Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	FMO5: In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. Belongs to the FMO family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.14.13.8; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450 Chromosomal Location of Human Ortholog: 1q21.1 Cellular Component: endoplasmic reticulum membrane; integral to membrane Molecular Function:FAD binding; NADP binding; flavin-containing monooxygenase activity
NCBI Summary:	Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
UniProt Code:	P49326
NCBI GenInfo Identifier:	1346021
NCBI Gene ID:	2330
NCBI Accession:	P49326.2
UniProt Secondary Accession:	P49326,P97872, Q8K4C0,
UniProt Related Accession:	P49326
Molecular Weight:	533
NCBI Full Name:	Dimethylaniline monooxygenase
NCBI Synonym Full Names:	flavin containing monooxygenase 5
NCBI Official Symbol:	FMO5
NCBI Protein Information:	dimethylaniline monooxygenase [N-oxide-forming] 5; dimethylaniline monooxygenase [N-oxide-forming] 5; FMO 5; dimethylaniline oxidase 5; hepatic flavin-containing monooxygenase 5
UniProt Protein Name:	Dimethylaniline monooxygenase [N-oxide-forming] 5
UniProt Synonym Protein Names:	Dimethylaniline oxidase 5; Hepatic flavin-containing monooxygenase 5; FMO 5
Protein Family:	Dimethylaniline monooxygenase
UniProt Gene Name:	FMO5
UniProt Entry Name:	FMO5_HUMAN

	Western blot analysis of extracts of various cell lines, using FMO5 antibody (CAB7673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using FMO5 antibody (CAB7673) at dilution of 1:100. Blue: DAPI for nuclear staining.