Cardiovascular Antibodies
Anti-FLT4 Antibody (CAB12332)
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- SKU:
- CAB12332
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-FLT4 Antibody |
| Antibody SKU: | CAB12332 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human FLT4 (NP_891555.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | LO2, A-549 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human FLT4 (NP_891555.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SQVS TMAL HIAQ ADAE DSPP SLQR HSLA ARYY NWVS FPGC LARG AETR GSSR MKTF EEFP MTPT TYKG SVDN QTDS GMVL ASEE FEQI ESRH RQES GFSC K |
| Gene ID: | 2324 |
| Uniprot: | P35916 |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus, Secreted, Single-pass type I membrane protein |
| Calculated MW: | 93kDa/145kDa/152kDa |
| Observed MW: | 180kDa |
| Synonyms: | FLT4, FLT-4, FLT41, LMPH1A, PCL, VEGFR-3, VEGFR3 |
| Background: | This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. |
| UniProt Protein Function: | VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; VEGFR family Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: plasma membrane; receptor complex Molecular Function:growth factor binding; protein binding; protein phosphatase binding; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity Biological Process: blood vessel morphogenesis; lymph vessel development; lymphangiogenesis; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; sprouting angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculature development Disease: Hemangioma, Capillary Infantile; Lymphedema, Hereditary, Ia |
| NCBI Summary: | This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35916 |
| NCBI GenInfo Identifier: | 357529070 |
| NCBI Gene ID: | 2324 |
| NCBI Accession: | P35916.3 |
| UniProt Secondary Accession: | P35916,Q16067, Q86W07, Q86W08, A8K6L4, B5A926, |
| UniProt Related Accession: | P35916,AAB23636 |
| Molecular Weight: | 153kDa |
| NCBI Full Name: | Vascular endothelial growth factor receptor 3 |
| NCBI Synonym Full Names: | fms related tyrosine kinase 4 |
| NCBI Official Symbol: | FLT4 |
| NCBI Official Synonym Symbols: | PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3 |
| NCBI Protein Information: | vascular endothelial growth factor receptor 3 |
| UniProt Protein Name: | Vascular endothelial growth factor receptor 3 |
| UniProt Synonym Protein Names: | Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4 |
| Protein Family: | Vascular endothelial growth factor receptor |
| UniProt Gene Name: | FLT4 |
 | Western blot analysis of extracts of various cell lines, using FLT4 antibody (CAB12332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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