Immunology Antibodies 1

Anti-FLT3 Antibody (CAB12437)

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SKU:
CAB12437
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-FLT3 Antibody
Antibody SKU:CAB12437
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 650-750 of human FLT3 (NP_004110.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, HL-60, LO2, Jurkat, Mouse testis
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 650-750 of human FLT3 (NP_004110.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ADSS EREA LMSE LKMM TQLG SHEN IVNL LGAC TLSG PIYL IFEY CCYG DLLN YLRS KREK FHRT WTEI FKEH NFSF YPTF QSHP NSSM PGSR EVQI HPDS D
Gene ID:2322
Uniprot:P36888
Cellular Location:Endoplasmic reticulum lumen, Membrane, Single-pass type I membrane protein
Calculated MW:108kDa/112kDa
Observed MW:125kDa
Synonyms:FLT3, CD135, FLK-2, FLK2, STK1
Background:This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
UniProt Protein Function:FLT3: a receptor tyrosine kinase of the PDGFR family that binds the FL cytokine. FLT3 -/- mice have an impaired developmental capacity of primitive hematopoietic progenitor cells of all lineages with the greatest impact on lymphopoietic precursors. Activating mutations found in one third of cases of acute myeloid leukemia (AML), as well as in acute lymphoblastic leukemia, acute promyelocytic leukemia and myelodysplastic syndrome. Inhibitors: Sutent and PKC412.
UniProt Protein Details:

Protein type:EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: endoplasmic reticulum; integral to plasma membrane; plasma membrane

Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; protein self-association; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: B cell differentiation; cytokine and chemokine mediated signaling pathway; hemopoiesis; leukocyte homeostasis; lymphocyte proliferation; myeloid progenitor cell differentiation; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of STAT protein; pro-B cell differentiation; protein amino acid autophosphorylation; regulation of apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Leukemia, Acute Lymphoblastic

NCBI Summary:This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]
UniProt Code:P36888
NCBI GenInfo Identifier:156630887
NCBI Gene ID:2322
NCBI Accession:P36888.2
UniProt Secondary Accession:P36888,Q13414, A0AVG9, B7ZLT7, B7ZLT8, F5H0A0,
UniProt Related Accession:P36888
Molecular Weight:113kDa
NCBI Full Name:Receptor-type tyrosine-protein kinase FLT3
NCBI Synonym Full Names:fms related tyrosine kinase 3
NCBI Official Symbol:FLT3  
NCBI Official Synonym Symbols:FLK2; STK1; CD135; FLK-2  
NCBI Protein Information:receptor-type tyrosine-protein kinase FLT3
UniProt Protein Name:Receptor-type tyrosine-protein kinase FLT3
UniProt Synonym Protein Names:FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD_antigen: CD135
Protein Family:Flt3 receptor-interacting lectin
UniProt Gene Name:FLT3  
Anti-FLT3 Antibody (CAB12437)Western blot analysis of extracts of various cell lines, using FLT3 antibody (CAB12437) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
Anti-FLT3 Antibody (CAB12437)Immunohistochemistry of paraffin-embedded rat pancreas using FLT3 antibody (CAB12437) at dilution of 1:100 (40x lens).
Anti-FLT3 Antibody (CAB12437)Immunohistochemistry of paraffin-embedded human liver cancer using FLT3 antibody (CAB12437) at dilution of 1:100 (40x lens).
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