Name: Anti-FLOT1 Antibody (CAB6220)
Brand: Cell Biology Antibodies 10
SKU: CAB6220
Availability: OutOfStock

Antibody Name:	Anti-FLOT1 Antibody
Antibody SKU:	CAB6220
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 128-427 of human FLOT1 (NP_005794.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HeLa, 293T, A-549, Mouse lung, Mouse heart, Mouse brain, Rat heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 128-427 of human FLOT1 (NP_005794.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SEQV FKVA SSDL VNMG ISVV SYTL KDIH DDQD YLHS LGKA RTAQ VQKD ARIG EAEA KRDA GIRE AKAK QEKV SAQY LSEI EMAK AQRD YELK KAAY DIEV NTRR AQAD LAYQ LQVA KTKQ QIEE QRVQ VQVV ERAQ QVAV QEQE IARR EKEL EARV RKPA EAER YKLE RLAE AEKS QLIM QAEA EAAS VRMR GEAE AFAI GARA RAEA EQMA KKAE AFQL YQEA AQLD MLLE KLPQ VAEE ISGP LTSA NKIT LVSS GSGT MGAA KVTG EVLD ILTR LPES VERL TGVS ISQV NHKP LRTA
Gene ID:	10211
Uniprot:	O75955
Cellular Location:	Cell membrane, Endosome, Melanosome, Membrane, Peripheral membrane protein, caveola
Calculated MW:	42kDa/47kDa
Observed MW:	47kDa

Synonyms:	FLOT1
Background:	This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	FLOT1: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. Belongs to the band 7/mec-2 family. Flotillin subfamily.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: signalosome; focal adhesion; membrane; lysosomal membrane; integral to membrane; melanosome; microtubule organizing center; caveola; flotillin complex; sarcolemma; endosome; lipid raft Molecular Function:ionotropic glutamate receptor binding; protein binding; protease binding Biological Process: extracellular matrix disassembly; axonogenesis; regulation of Rho protein signal transduction
NCBI Summary:	Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	O75955
NCBI GenInfo Identifier:	26006960
NCBI Gene ID:	10211
NCBI Accession:	O75955.3
UniProt Secondary Accession:	O75955,Q969J8, Q9UHW1, Q9UNV8, B4DVY7,
UniProt Related Accession:	O75955
Molecular Weight:	42,080 Da
NCBI Full Name:	Flotillin-1
NCBI Synonym Full Names:	flotillin 1
NCBI Official Symbol:	FLOT1
NCBI Protein Information:	flotillin-1; integral membrane component of caveolae
UniProt Protein Name:	Flotillin-1
Protein Family:	Flotillin-like protein
UniProt Gene Name:	FLOT1
UniProt Entry Name:	FLOT1_HUMAN

	Western blot analysis of extracts of various cell lines, using FLOT1 antibody (CAB6220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse kidney using FLOT1 Rabbit pAb (CAB6220) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using FLOT1 Rabbit pAb (CAB6220) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using FLOT1 antibody (CAB6220). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug FLOT1 antibody (CAB6220). Western blot was performed from the immunoprecipitate using FLOT1 antibody (CAB6220) at a dilition of 1:1000.