Epigenetics & Nuclear Signaling Antibodies 1

Anti-FLJ12529 Antibody (CAB13880)

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SKU:
CAB13880
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-FLJ12529 Antibody
Antibody SKU:CAB13880
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-240 of human FLJ12529 (NP_079087.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, HepG2, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-240 of human FLJ12529 (NP_079087.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LIDI YADE EFNQ DPEF NNTD QIDL YDDV LTAT SQPS DDRS SSTE PPPP VRQE PSPK PNNK TPAI LYTY SGLR NRRA AVYV GSFS WWTT DQQL IQVI RSIG VYDV VELK FAEN RANG QSKG YAEV VVAS ENSV HKLL ELLP GKVL NGEK VDVR PATR QNLS QFEA QARK RECV RVPR GGIP PRAH SRDS SDS
Gene ID:79869
Uniprot:Q8N684
Cellular Location:Nucleus
Calculated MW:51kDa/52kDa/56kDa
Observed MW:60kDa
Synonyms:CPSF7, CFIm59, FLJ12529
Background:Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is composed of three different subunits of 25, 59, and 68 kDa, and it functions as a heterotetramer, with a dimer of the 25 kDa subunit binding to two of the 59 or 68 kDa subunits. The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes.
UniProt Protein Function:CPSF7: probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. Probable component of the cleavage factor Im (CFIm) complex, composed at least of NUDT21/CPSF5 and CPSF6 or CPSF7. Two alternatively-spliced isoforms have been described.
UniProt Protein Details:

Protein type:RNA splicing; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; membrane; nucleus; mRNA cleavage factor complex

Molecular Function:protein binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; protein tetramerization

UniProt Code:Q8N684
NCBI GenInfo Identifier:74759932
NCBI Gene ID:79869
NCBI Accession:Q8N684.1
UniProt Secondary Accession:Q8N684,Q7Z3H9, Q9H025, Q9H9V1, B3KU04, C9K0Q4,
UniProt Related Accession:Q8N684
Molecular Weight:471
NCBI Full Name:Cleavage and polyadenylation specificity factor subunit 7
NCBI Synonym Full Names:cleavage and polyadenylation specific factor 7, 59kDa
NCBI Official Symbol:CPSF7  
NCBI Official Synonym Symbols:CFIm59  
NCBI Protein Information:cleavage and polyadenylation specificity factor subunit 7; CPSF 59 kDa subunit; cleavage factor Im complex 59 kDa subunit; pre mRNA cleavage factor I, 59 kDa subunit; pre-mRNA cleavage factor I, 59 kDa subunit; pre-mRNA cleavage factor Im 59 kDa subunit;
UniProt Protein Name:Cleavage and polyadenylation specificity factor subunit 7
UniProt Synonym Protein Names:Cleavage and polyadenylation specificity factor 59 kDa subunit; CFIm59; CPSF 59 kDa subunit; Pre-mRNA cleavage factor Im 59 kDa subunit
Protein Family:Cleavage and polyadenylation specificity factor
UniProt Gene Name:CPSF7  
UniProt Entry Name:CPSF7_HUMAN
Anti-FLJ12529 Antibody (CAB13880)Western blot analysis of extracts of various cell lines, using CPSF7 antibody (CAB13880) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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