Anti-FLII Antibody (CAB15273)

Name: Anti-FLII Antibody (CAB15273)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB15273
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Cell Biology Antibodies 1

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Cell Biology Antibodies 14

Antibody Name:	Anti-FLII Antibody
Antibody SKU:	CAB15273
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LRLA GASP ATVA AAAA AGSG PKDP MARK MRLR RRKD SAQD DQAK QVLK GMSD VAQE KNKK QEES ADAR APSG KVRR WDQG LEKP RLDY SEFF TEDV GQLP GLTI WQIE NFVP VLVE EAFH GKFY EADC YIVL KTFL DDSG SLNW EIYY WIGG EATL DKKA CSAI HAVN LRNY LGAE CRTV REEM GDES EEFL QVFD NDIS YIEG GTAS GFYT VEDT HYVT RMYR VYGK KNIK LEPV PLKG TSLD PRFV FLLD RGLD IYVW R
Gene ID:	2314
Uniprot:	Q13045
Cellular Location:	Cell junction, Cytoplasm, Nucleus, centrosome, cytoskeleton, focal adhesion, microtubule organizing center
Calculated MW:	138kDa/143kDa/144kDa
Observed MW:	160kDa

Synonyms:	FLII, FLI, FLIL, Fli1
Background:	This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

UniProt Protein Function:	May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
NCBI Summary:	This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13045
NCBI GenInfo Identifier:	372266127
NCBI Gene ID:	2314
NCBI Accession:	NP_001243193.1
UniProt Secondary Accession:	Q13045,B4DIL0, F5H407, J3QLG3,
UniProt Related Accession:	Q13045
Molecular Weight:	143,652 Da
NCBI Full Name:	protein flightless-1 homolog isoform 2
NCBI Synonym Full Names:	FLII, actin remodeling protein
NCBI Official Symbol:	FLII
NCBI Official Synonym Symbols:	FLI; FLIL; Fli1
NCBI Protein Information:	protein flightless-1 homolog
UniProt Protein Name:	Protein flightless-1 homolog
Protein Family:	Flagellum-specific ATP synthase
UniProt Gene Name:	FLII

	Western blot analysis of extracts of various cell lines, using FLII antibody (CAB15273) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5S.
	Immunohistochemistry of paraffin-embedded rat heart using FLII antibody (CAB15273) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using FLII antibody (CAB15273) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using FLII antibody (CAB15273) at dilution of 1:100 (40x lens).