Epigenetics & Nuclear Signaling Antibodies 2

Anti-FLI1 Antibody (CAB5644)

(No reviews yet) Write a Review
SKU:
CAB5644
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-FLI1 Antibody
Antibody SKU:CAB5644
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDGT IKEA LSVV SDDQ SLFD SAYG AAAH LPKA DMTA SGSP DYGQ PHKI NPLP PQQE WINQ PVRV NVKR EYDH MNGS RESP VDCS VSKC SKLV GGGE SNPM
Gene ID:2313
Uniprot:Q01543
Cellular Location:Nucleus
Calculated MW:29kDa/43kDa/47kDa/50kDa
Observed MW:53kDa
Synonyms:FLI1, EWSR2, SIC-1, BDPLT21
Background:This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 11q24.1-q24.3

Cellular Component: nucleus

Molecular Function:DNA binding; chromatin binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

NCBI Summary:This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:Q01543
NCBI GenInfo Identifier:399496
NCBI Gene ID:2313
NCBI Accession:Q01543.1
UniProt Secondary Accession:Q01543,Q14319, Q92480, Q9UE07, B2R8H2, B4DFV4, B4DTC6 G3V183,
UniProt Related Accession:Q01543
Molecular Weight:
NCBI Full Name:Friend leukemia integration 1 transcription factor
NCBI Synonym Full Names:Fli-1 proto-oncogene, ETS transcription factor
NCBI Official Symbol:FLI1  
NCBI Official Synonym Symbols:EWSR2; SIC-1  
NCBI Protein Information:Friend leukemia integration 1 transcription factor; transcription factor ERGB; Ewing sarcoma breakpoint region 2; Friend leukemia virus integration 1
UniProt Protein Name:Friend leukemia integration 1 transcription factor
UniProt Synonym Protein Names:Proto-oncogene Fli-1; Transcription factor ERGB
Protein Family:Friend leukemia integration 1 transcription factor
UniProt Gene Name:FLI1  
UniProt Entry Name:FLI1_HUMAN
Anti-FLI1 Antibody (CAB5644)Western blot analysis of extracts of Jurkat cells, using FLI1 antibody (CAB5644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-FLI1 Antibody (CAB5644)Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody (CAB5644) at dilution of 1:100 (40x lens).
Anti-FLI1 Antibody (CAB5644)Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody (CAB5644) at dilution of 1:100 (40x lens).
Anti-FLI1 Antibody (CAB5644)Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug FLI1 antibody (CAB5644). Western blot was performed from the immunoprecipitate using FLI1 antibody (CAB5644) at a dilition of 1:1000.
View AllClose

0 Reviews

View AllClose

Related Products