Cell Biology Antibodies 4

Anti-FLAD1 Antibody (CAB14293)

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SKU:
CAB14293
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FLAD1 Antibody
Antibody SKU:CAB14293
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 388-587 of human FLAD1 (NP_079483.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 388-587 of human FLAD1 (NP_079483.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ETSL AQYS LTQL CVGF NGGK DCTA LLHL FHAA VQRK LPDV PNPL QILY IRSI SPFP ELEQ FLQD TIKR YNLQ MLEA EGSM KQAL GELQ ARHP QLEA VLMG TRRT DPYS CSLC PFSP TDPG WPAF MRIN PLLD WTYR DIWD FLRQ LFVP YCIL YDRG YTSL GSRE NTVR NPAL KCLS PGGH PTYR PAYL LENE EEER NSRT
Gene ID:80308
Uniprot:Q8NFF5
Cellular Location:Cytoplasm, Mitochondrion matrix
Calculated MW:32kDa/36kDa/49kDa/54kDa/65kDa
Observed MW:65kDa
Synonyms:FLAD1, FAD1, FADS, LSMFLAD, PP591
Background:This gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
UniProt Protein Function:FLAD1: Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - riboflavin; EC 2.7.7.2; Transferase

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function:FMN adenylyltransferase activity; protein binding

Biological Process: riboflavin metabolic process

NCBI Summary:This gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:Q8NFF5
NCBI GenInfo Identifier:74751275
NCBI Gene ID:80308
NCBI Accession:Q8NFF5.1
UniProt Secondary Accession:Q8NFF5,Q8N5J1, Q8N686, Q8WU93, Q8WUJ4, Q96CR8, Q99764 Q9HBN6,
UniProt Related Accession:Q8NFF5
Molecular Weight:36,865 Da
NCBI Full Name:FAD synthase
NCBI Synonym Full Names:flavin adenine dinucleotide synthetase 1
NCBI Official Symbol:FLAD1  
NCBI Official Synonym Symbols:FAD1; FADS; PP591; LSMFLAD  
NCBI Protein Information:FAD synthase
UniProt Protein Name:FAD synthase
UniProt Synonym Protein Names:FAD pyrophosphorylase; FMN adenylyltransferase; Flavin adenine dinucleotide synthaseIncluding the following 2 domains:Molybdenum cofactor biosynthesis protein-like regionFAD synthase region
UniProt Gene Name:FLAD1  
UniProt Entry Name:FAD1_HUMAN
Anti-FLAD1 Antibody (CAB14293)Western blot analysis of extracts of various cell lines, using FLAD1 antibody (CAB14293) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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